A ratiometric fluorescent probe for the detection of biological thiols based on a new supramolecular design

被引:2
|
作者
Jiang, Siyang [1 ,2 ,3 ]
Wang, Shuyi [1 ,2 ,3 ]
Zhao, Zizhen [1 ,2 ,3 ]
Ma, Da [1 ,2 ]
机构
[1] Taizhou Univ, Sch Pharmaceut Engn, Jiaojiang 318000, Zhejiang, Peoples R China
[2] Taizhou Univ, Inst Adv Studies, Jiaojiang 318000, Zhejiang, Peoples R China
[3] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
基金
中国国家自然科学基金;
关键词
Fluorescent probe; Host -guest chemistry; Biological thiols; Ratiometric detection; CANCER; DISCRIMINATION; GLUTATHIONE; CYSTEINE; PLASMA; CYS;
D O I
10.1016/j.saa.2023.123167
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
A new ratiometric fluorescent probe is designed and prepared based on the concept of supramolecular encapsulation and dye competition. This supramolecular probe is based on two commercially-available dyes, one common guest and a simple-to-synthesize host. Fluorescence spectroscopy confirms that the supramolecular probe is capable of detecting thiols quantitatively with a broad linear region in phosphate buffered saline or fetal bovine serum. Mechanistic study shows a reaction between thiol specie and the guest to alter the distribution of encapsulated dyes. The supramolecular probes are demonstrated to quantitatively detect extracellular biological thiols by plate reader, which shows it keeps its effectiveness in complex buffered systems.
引用
收藏
页数:7
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