The two-step cargo recognition mechanism of heterotrimeric kinesin

被引：1

作者：

Jiang, Xuguang ^{[1
,2
]}

Ogawa, Tadayuki ^{[1
,3
,4
]}

Yonezawa, Kento ^{[5
,6
]}

Shimizu, Nobutaka ^{[5
]}

Ichinose, Sotaro ^{[1
,7
]}

Uchihashi, Takayuki ^{[8
,9
]}

Nagaike, Wataru ^{[8
]}

Moriya, Toshio ^{[5
]}

Adachi, Naruhiko ^{[5
]}

Kawasaki, Masato ^{[5
]}

Dohmae, Naoshi ^{[4
]}

Senda, Toshiya ^{[5
]}

Hirokawa, Nobutaka ^{[1
,10
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Cell Biol & Anat, Tokyo, Japan

[2] Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, Beijing, Peoples R China

[3] Dokkyo Med Univ, Res Ctr Adv Med Sci, Tochigi, Japan

[4] RIKEN Ctr Sustainable Resource Sci, Biomol Characterizat Unit, Wako, Japan

[5] High Energy Accelerator Res Org KEK, Inst Mat Struct Sci, Struct Biol Res Ctr, Photon Factory, Tsukuba, Japan

[6] Nara Inst Sci & Technol, Ctr Digital Green Innovat, Nara, Japan

[7] Gunma Univ, Grad Sch Med, Dept Anat, Gunma, Japan

[8] Nagoya Univ, Dept Phys, Nagoya, Japan

[9] Natl Inst Nat Sci, Exploratory Res Ctr Life & Living Syst ExCELLS, Okazaki, Japan

[10] Juntendo Univ, Juntendo Adv Res Inst Hlth Sci, Tokyo, Japan

来源：

EMBO REPORTS | 2023年 / 24卷 / 11期

基金：

日本学术振兴会;

关键词：

Cargo binding; Intracellular transport; KIF3; Kinesin; Protein dynamics; LEFT-RIGHT ASYMMETRY; SUPERFAMILY PROTEIN; HETEROMERIC KINESIN; MOLECULAR MOTORS; TRANSPORT; KIF3A; FAMILY; CILIA; INTERACTS; AXONEMES;

D O I：

10.15252/embr.202356864

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kinesin-driven intracellular transport is essential for various cell biological events and thus plays a crucial role in many pathological processes. However, little is known about the molecular basis of the specific and dynamic cargo-binding mechanism of kinesins. Here, an integrated structural analysis of the KIF3/KAP3 and KIF3/KAP3-APC complexes unveils the mechanism by which KIF3/KAP3 can dynamically grasp APC in a two-step manner, which suggests kinesin-cargo recognition dynamics composed of cargo loading, locking, and release. Our finding is the first demonstration of the two-step cargo recognition and stabilization mechanism of kinesins, which provides novel insights into the intracellular trafficking machinery.

引用

页数：14

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