Heterologous 1,3-Propanediol Production Using Different Recombinant Clostridium beijerinckii DSM 6423 Strains

被引:5
|
作者
Schoch, Teresa [1 ]
Baur, Tina [1 ]
Kunz, Johanna [1 ]
Stoeferle, Sophia [1 ]
Duerre, Peter [1 ]
机构
[1] Univ Ulm, Inst Mikrobiol & Biotechnol, D-89081 Ulm, Germany
关键词
Clostridium beijerinckii; Clostridium pasteurianum; Clostridium diolis; 1; 3-propanediol production; glycerol fermentation; promoter effects; B-12-DEPENDENT GLYCEROL DEHYDRATASE; GENE-EXPRESSION; ANAEROBIC FERMENTATION; KLEBSIELLA-PNEUMONIAE; CRUDE GLYCEROL; PASTEURIANUM; CONVERSION; SYSTEM; ACETOBUTYLICUM; PLATFORM;
D O I
10.3390/microorganisms11030784
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
1,3-propanediol (1,3-PDO) is a valuable basic chemical, especially in the polymer industry to produce polytrimethylene terephthalate. Unfortunately, the production of 1,3-PDO mainly depends on petroleum products as precursors. Furthermore, the chemical routes have significant disadvantages, such as environmental issues. An alternative is the biobased fermentation of 1,3-PDO from cheap glycerol. Clostridium beijerinckii DSM 6423 was originally reported to produce 1,3-PDO. However, this could not be confirmed, and a genome analysis revealed the loss of an essential gene. Thus, 1,3-PDO production was genetically reinstalled. Genes for 1,3-PDO production from Clostridium pasteurianum DSM 525 and Clostridium beijerinckii DSM 15410 (formerly Clostridium diolis) were introduced into C. beijerinckii DSM 6423 to enable 1,3-PDO production from glycerol. 1,3-PDO production by recombinant C. beijerinckii strains were investigated under different growth conditions. 1,3-PDO production was only observed for C. beijerinckii [pMTL83251_Ppta-ack_1,3-PDO.diolis], which harbors the genes of C. beijerinckii DSM 15410. By buffering the growth medium, production could be increased by 74%. Furthermore, the effect of four different promoters was analyzed. The use of the constitutive thlA promoter from Clostridium acetobutylicum led to a 167% increase in 1,3-PDO production compared to the initial recombinant approach.
引用
收藏
页数:16
相关论文
共 50 条
  • [21] Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
    Zhu, Jian-Guo
    Li, Shuang
    Ji, Xiao-Jun
    Huang, He
    Hu, Nan
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2009, 25 (07): : 1217 - 1223
  • [22] Microbial production of 1,3-propanediol
    Biebl, H
    Menzel, K
    Zeng, AP
    Deckwer, WD
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 52 (03) : 289 - 297
  • [23] Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410
    Sedlar, Karel
    Vasylkivska, Maryna
    Musilova, Jana
    Branska, Barbora
    Provaznik, Ivo
    Patakova, Petra
    GENOMICS, 2021, 113 (01) : 1109 - 1119
  • [24] Biotechnological synthesis of 1,3-propanediol using Clostridium ssp.
    Leja, Katarzyna
    Czaczyk, Katarzyna
    Myszka, Kamila
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (54): : 11093 - 11101
  • [25] Microbial production of 1,3-propanediol
    H. Biebl
    K. Menzel
    A.-P. Zeng
    W.-D. Deckwer
    Applied Microbiology and Biotechnology, 1999, 52 : 289 - 297
  • [26] Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized Clostridium butyricum
    Dolejs, Igor
    Liskova, Monika
    Krasnan, Vladimir
    Markosova, Kristina
    Rosenberg, Michal
    Lorenzini, Fabio
    Marr, Andrew C.
    Rebros, Martin
    CATALYSTS, 2019, 9 (04):
  • [27] Production of 1,3-Propanediol by Clostridium butyricum Growing on Biodiesel Derived Glycerol
    Martins, Fernanda F.
    Saab, Vanessa S.
    Ribeiro, Claudia M. S.
    Coelho, Maria Alice Z.
    Ferreira, Tatiana F.
    2ND INTERNATIONAL CONFERENCE ON BIOMASS (ICONBM 2016), 2016, 50 : 289 - 294
  • [28] Effect of cassava pulp supplement on 1,3-propanediol production by Clostridium butyricum
    Apiwatanapiwat, Waraporn
    Vaithanomsat, Pilanee
    Tachaapaikoon, Chakrit
    Ratanakhanokchai, Khanok
    Kosugi, Akihiko
    JOURNAL OF BIOTECHNOLOGY, 2016, 230 : 44 - 46
  • [29] Cellulose hydrolysis and IBE fermentation of eucalyptus sawdust for enhanced biobutanol production by Clostridium beijerinckii DSM 6423
    Cebreiros, Florencia
    Daniel Ferrari, Mario
    Lareo, Claudia
    INDUSTRIAL CROPS AND PRODUCTS, 2019, 134 : 50 - 61
  • [30] Production of 1,3-propanediol by Clostridium butyricum in continuous culture with cell recycling
    Reimann, A
    Biebl, H
    Deckwer, WD
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 49 (04) : 359 - 363