Heterologous 1,3-Propanediol Production Using Different Recombinant Clostridium beijerinckii DSM 6423 Strains

被引:5
|
作者
Schoch, Teresa [1 ]
Baur, Tina [1 ]
Kunz, Johanna [1 ]
Stoeferle, Sophia [1 ]
Duerre, Peter [1 ]
机构
[1] Univ Ulm, Inst Mikrobiol & Biotechnol, D-89081 Ulm, Germany
关键词
Clostridium beijerinckii; Clostridium pasteurianum; Clostridium diolis; 1; 3-propanediol production; glycerol fermentation; promoter effects; B-12-DEPENDENT GLYCEROL DEHYDRATASE; GENE-EXPRESSION; ANAEROBIC FERMENTATION; KLEBSIELLA-PNEUMONIAE; CRUDE GLYCEROL; PASTEURIANUM; CONVERSION; SYSTEM; ACETOBUTYLICUM; PLATFORM;
D O I
10.3390/microorganisms11030784
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
1,3-propanediol (1,3-PDO) is a valuable basic chemical, especially in the polymer industry to produce polytrimethylene terephthalate. Unfortunately, the production of 1,3-PDO mainly depends on petroleum products as precursors. Furthermore, the chemical routes have significant disadvantages, such as environmental issues. An alternative is the biobased fermentation of 1,3-PDO from cheap glycerol. Clostridium beijerinckii DSM 6423 was originally reported to produce 1,3-PDO. However, this could not be confirmed, and a genome analysis revealed the loss of an essential gene. Thus, 1,3-PDO production was genetically reinstalled. Genes for 1,3-PDO production from Clostridium pasteurianum DSM 525 and Clostridium beijerinckii DSM 15410 (formerly Clostridium diolis) were introduced into C. beijerinckii DSM 6423 to enable 1,3-PDO production from glycerol. 1,3-PDO production by recombinant C. beijerinckii strains were investigated under different growth conditions. 1,3-PDO production was only observed for C. beijerinckii [pMTL83251_Ppta-ack_1,3-PDO.diolis], which harbors the genes of C. beijerinckii DSM 15410. By buffering the growth medium, production could be increased by 74%. Furthermore, the effect of four different promoters was analyzed. The use of the constitutive thlA promoter from Clostridium acetobutylicum led to a 167% increase in 1,3-PDO production compared to the initial recombinant approach.
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页数:16
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