Recent Advances in β-Glucosidase Sequence and Structure Engineering: A Brief Review

被引：10

作者：

Ouyang, Bei ^{[1
]}

Wang, Guoping ^{[1
]}

Zhang, Nian ^{[1
]}

Zuo, Jiali ^{[2
]}

Huang, Yunhong ^{[1
]}

Zhao, Xihua ^{[1
]}

机构：

[1] Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Peoples R China

[2] Jiangxi Normal Univ, Sch Comp & Informat Engn, Nanchang 330022, Peoples R China

来源：

MOLECULES | 2023年 / 28卷 / 13期

基金：

中国国家自然科学基金;

关键词：

beta-glucosidase; biocatalysis; biosynthesis; enzyme engineering; MOLECULAR-DYNAMICS; HALOTHERMOTHRIX-ORENII; RATIONAL DESIGN; MUTAGENESIS; TOLERANCE; STABILITY; ENZYMES; DOCKING; THERMOSTABILITY; TEMPERATURE;

D O I：

10.3390/molecules28134990

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-glucosidases (BGLs) play a crucial role in the degradation of lignocellulosic biomass as well as in industrial applications such as pharmaceuticals, foods, and flavors. However, the application of BGLs has been largely hindered by issues such as low enzyme activity, product inhibition, low stability, etc. Many approaches have been developed to engineer BGLs to improve these enzymatic characteristics to facilitate industrial production. In this article, we review the recent advances in BGL engineering in the field, including the efforts from our laboratory. We summarize and discuss the BGL engineering studies according to the targeted functions as well as the specific strategies used for BGL engineering.

引用

页数：18

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