High titers of infectious SARS-CoV-2 in corpses of patients with COVID-19

被引：3

作者：

Saitoh, Hisako ^{[1
]}

Sakai-Tagawa, Yuko ^{[2
]}

Nagasawa, Sayaka ^{[1
]}

Torimitsu, Suguru ^{[1
,3
]}

Kubota, Kazumi ^{[4
]}

Hirata, Yuichiro ^{[1
,5
]}

Iwatsuki-Horimoto, Kiyoko ^{[2
]}

Motomura, Ayumi ^{[1
,6
]}

Ishii, Namiko ^{[1
,6
]}

Okaba, Keisuke ^{[1
,6
]}

Horioka, Kie ^{[6
]}

Abe, Hiroyuki ^{[7
]}

Ikemura, Masako ^{[7
]}

Rokutan, Hirofumi ^{[7
]}

Hinata, Munetoshi ^{[7
]}

Iwasaki, Akiko ^{[7
]}

Yasunaga, Yoichi ^{[7
]}

Nakajima, Makoto ^{[3
]}

Yamaguchi, Rutsuko ^{[1
,3
]}

Tsuneya, Shigeki ^{[1
,3
]}

Kira, Kei ^{[1
,3
]}

Kobayashi, Susumu ^{[1
,3
]}

Inokuchi, Go ^{[1
,3
]}

Chiba, Fumiko ^{[1
,3
]}

Hoshioka, Yumi ^{[1
]}

Mori, Aika ^{[1
]}

Yamamoto, Isao ^{[8
,9
]}

Nakagawa, Kimiko ^{[8
,9
]}

Katano, Harutaka ^{[5
]}

Iida, Shun ^{[5
]}

Suzuki, Tadaki ^{[5
]}

Akitomi, Shinji ^{[3
,10
]}

Hasegawa, Iwao ^{[8
,9
]}

Ushiku, Tetsuo ^{[7
]}

Yajima, Daisuke ^{[1
,6
]}

Iwase, Hirotaro ^{[1
,3
]}

Makino, Yohsuke ^{[1
,3
]}

Kawaoka, Yoshihiro ^{[2
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Legal Med, Chiba, Japan

[2] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Virol, Tokyo, Japan

[3] Univ Tokyo, Grad Sch Med, Dept Forens Med, Tokyo, Japan

[4] Univ Tokyo Hosp, Dept Healthcare Informat Management, Tokyo, Japan

[5] Natl Inst Infect Dis, Dept Pathol, Tokyo, Japan

[6] Int Univ Hlth & Welf, Dept Legal Med, Tokyo, Japan

[7] Univ Tokyo, Grad Sch Med, Dept Pathol, Tokyo, Japan

[8] Kanagawa Dent Univ, Dept Forens Med, Yokosuka, Japan

[9] Publ Interest Inc Assoc Nihon Kousei Kyoukai, Yokosuka, Japan

[10] Japan Med Assoc, Res Inst, Tokyo, Japan

来源：

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES | 2023年 / 129卷

关键词：

SARS-CoV-2; COVID-19; Infectious titer; Virus isolation; Autopsy; Postmortem interval;

D O I：

10.1016/j.ijid.2023.01.046

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: The prolonged presence of infectious SARS-CoV-2 in deceased patients with COVID-19 has been reported. However, infectious virus titers have not been determined. Such information is important for public health, death investigation, and handling corpses. The aim of this study was to assess the level of SARS-CoV-2 infectivity in the corpses of patients with COVID-19.Methods: We collected 11 nasopharyngeal swabs and 19 lung tissue specimens from 11 autopsy cases with COVID-19 in 2021. We then investigated the viral genomic copy number by real-time reverse transcription-polymerase chain reaction and infectious titers by cell culture and virus isolation.Results: Infectious virus was present in six of 11 (55%) cases, four of 11 (36%) nasopharyngeal swabs, and nine of 19 (47%) lung specimens. The virus titers ranged from 6.00E + 01 plaque-forming units/ml to 2.09E + 06 plaque-forming units/g. In all cases in which an infectious virus was found, the time from death to discovery was within 1 day and the longest postmortem interval was 13 days.Conclusion: The corpses of patients with COVID-19 may have high titers of infectious virus after a long postmortem interval (up to 13 days). Therefore, appropriate infection control measures must be taken when handling corpses.(c) 2023 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )

引用

页码：103 / 109

页数：7

共 50 条

[21] Characteristics of SARS-CoV-2 and COVID-19
Ben Hu
Hua Guo
Peng Zhou
Zheng-Li Shi
Nature Reviews Microbiology, 2021, 19 : 141 - 154
[22] Dementia and SARS-Cov-2/COVID-19
Zieschang, T.
ZEITSCHRIFT FUR GERONTOLOGIE UND GERIATRIE, 2021, 54 (SUPPL 1): : S14 - S14
[23] SARS-CoV-2 (COVID-19) by the numbers
Bar-On, Yinon M.
Flamholz, Avi
Phillips, Rob
Milo, Ron
ELIFE, 2020, 9
[24] SARS-CoV-2, COVID-19, and the Eye
Cunningham, Emmett T.
Zierhut, Manfred
OCULAR IMMUNOLOGY AND INFLAMMATION, 2021, 29 (04) : 629 - 630
[25] Delirium and SARS-CoV-2/COVID-19
von Arnim, C.
ZEITSCHRIFT FUR GERONTOLOGIE UND GERIATRIE, 2021, 54 (SUPPL 1): : S14 - S14
[26] Characteristics of SARS-CoV-2 and COVID-19
Hu, Ben
Guo, Hua
Zhou, Peng
Shi, Zheng-Li
NATURE REVIEWS MICROBIOLOGY, 2021, 19 (03) : 141 - 154
[27] SARS-CoV-2, COVID-19 and Neurodegeneration
Toenges, Lars
Klebe, Stephan
BRAIN SCIENCES, 2022, 12 (07)
[28] SARS-CoV-2 and COVID-19 for the ophthalmologist
Brandao-de-Resende, Camilo
Diniz-Filho, Alberto
de Almeida Brito, Fabiano
Vasconcelos-Santos, Daniel Vitor
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 2021, 49 (01): : 70 - 80
[29] IgG antibody titers against SARS-CoV-2 nucleocapsid protein correlate with the severity of COVID-19 patients
Yang, Li
Xu, Qiang
Yang, Bin
Li, Jiayu
Dong, Rong
Da, Jingjing
Ye, Zhixu
Xu, Yongjie
Zhou, Hourong
Zhang, Xiangyan
Liu, Lin
Zha, Yan
Yu, Fuxun
BMC MICROBIOLOGY, 2021, 21 (01)
[30] IgG antibody titers against SARS-CoV-2 nucleocapsid protein correlate with the severity of COVID-19 patients
Li Yang
Qiang Xu
Bin Yang
Jiayu Li
Rong Dong
Jingjing Da
Zhixu Ye
Yongjie Xu
Hourong Zhou
Xiangyan Zhang
Lin Liu
Yan Zha
Fuxun Yu
BMC Microbiology, 21

← 1 2 3 4 5 →