Bacterial diversity of biofilms on polyhydroxybutyrate exposed to marine conditions: Ex-situ vs. in-situ tests

被引：0

作者：

Bhalerao, Aniruddha ^{[1
]}

Dueker, Urda ^{[1
]}

Weber, Miriam ^{[2
]}

Eich, Andreas ^{[2
]}

Lott, Christian ^{[2
]}

Endres, Hans Josef ^{[3
]}

Nogueira, Regina ^{[1
]}

机构：

[1] Leibniz Univ Hannover, Inst Sanit Engn & Waste Management, Welfengarten 1, D-30167 Hannover, Germany

[2] HYDRA Marine Sci GmbH, Steinfeldweg 15, D-77815 Buhl, Germany

[3] Leibniz Univ Hannover, Inst Plast & Circulat Technol, An der Univ 2, D-30823 Hannover, Germany

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2023年 / 905卷

关键词：

Polyhydroxybutyrate; Biofilm; Bacterial diversity; Marine conditions; In-situ test; Ex-situ test; MICROBIAL-DEGRADATION; POLYHYDROXYALKANOATES; STRATEGY;

D O I：

10.1016/j.scitotenv.2023.167458

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Biofilms form on any available surface and, depending on the characteristics of the material and the environmental conditions, biodegradation can take place. We compared the bacterial composition of polyhydroxybutyrate (PHB)-related biofilm communities from marine ex-situ and in-situ tests to assess the differences in diversity and abundance between these two biofilms. This comparison will help to better assess the transferability of tank tests to real-life scenarios. The in-situ tests were set up in the Mediterranean Sea on the Island of Elba, Italy where PHB-tensile bars were lodged in the sediments. This created a water-exposed aerobic and mud-planted anaerobic scenario. The ex-situ tests were modeled after in-situ tests and performed in temperature-controlled tanks. The PHB-related biofilms were harvested after 240 days of exposure along with planktonic bacteria, and particle- and sediment-related biofilm. The bacterial composition was elucidated using 16S rDNA sequencing. Biofilms harvested from the in-situ test were more diverse, less even, and contained more rare species compared to biofilms from the ex-situ test. The PHB-related biofilm was characterized by a higher abundance of the bacterial order Desulfobacterales. The composition of PHB-related biofilm varied significantly between the two tests and between aerobic and anaerobic conditions. The composition of PHB-related biofilm was significantly different from planktonic bacteria, particle, and sediment-related biofilm, showing the influence of PHB on the biofilm composition. Thus, the ex-situ tank test for PHB degradation cannot, in terms of bacterial composition, simulate the in-situ conditions to their full extent.

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页数：12