Peptide encoded by lncRNA BVES-AS1 promotes cell viability, migration, and invasion in colorectal cancer cells via the SRC/mTOR signaling pathway

被引：15
|
作者：
Zheng, Weiwei [1 ]
Guo, Yingchang [2 ]
Zhang, Guangtan [1 ]
Bai, Junwei [1 ]
Song, Yucheng [1 ]
Song, Xiaofei [1 ]
Zhu, Qinhui [3 ]
Bao, Xuebin [1 ]
Wu, Gang [1 ]
Zhang, Chao [1 ]
机构：
[1] Henan Univ, Peoples Hosp, Henan Prov Peoples Hosp, Sch Clin Med,Dept Gastrointestinal Surg,Zhengzhou, Zhengzhou, Henan Province, Peoples R China
[2] Xinxiang Med Coll, Dept Intervent Therapy, Affiliated Hosp 1, Xinxiang, Henan Province, Peoples R China
[3] Shangcai Peoples Hosp, Dept Gen Surg, Zhumadian, Henan Province, Peoples R China
来源：
PLOS ONE | 2023年 / 18卷 / 06期
关键词：
PROLIFERATION;
D O I：
10.1371/journal.pone.0287133
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Long non-coding RNAs (lncRNAs) have been revealed to harbor open reading frames (ORFs) that can be translated into small peptides. The peptides may participate in the pathogenesis of colorectal cancer (CRC). Herein, we investigated the role of a lncRNA BVES-AS1-encoded peptide in colorectal tumorigenesis. Through bioinformatic analysis, lncRNA BVES-AS1 was predicted to have encoding potential and to be associated with poor prognosis of patients with CRC. In CRC cells, BVES-AS1 was validated to encode a 50-aa-length micro-peptide, named BVES-AS1-201-50aa, through a western blotting method. BVES-AS1-201-50aa enhanced cell viability and promoted the migratory and invasive capacities of HCT116 and SW480 CRC cells in vitro, validated via CCK-8 assay and transwell assay, respectively. Immunofluorescence assay showed that BVES-AS1-201-50aa increased the expression of proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase 9 (MMP9) in CRC cells. We further verified that BVES-AS1-201-50aa targeted and activated the Src/mTOR signaling pathway in CRC cells by co-immunoprecipitation (Co-IP) experiment, qualitative proteomic analysis, and western blotting. Our findings demonstrated that BVES-AS1 could encode a micro-peptide, which promoted CRC cell viability, migration, and invasion in vitro. Our current work broadens the diversity and breadth of lncRNAs in human carcinogenesis.
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页数：14
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