Sclerotinia sclerotiorum Protoplast Preparation and Transformation

被引：1
|
作者：
Lan, Chi [1 ,2 ]
Qiao, Lulu [1 ,2 ,3 ]
Niu, Dongdong [1 ,2 ]
机构：
[1] Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing 210095, Peoples R China
[2] Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Peoples R China
[3] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou, Peoples R China
来源：
BIO-PROTOCOL | 2023年 / 13卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Sclerotinia sclerotiorum; Protoplast; Preparation; Transformation; Gene function; Mutant;
D O I：
10.21769/BioProtoc.4581
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Sclerotinia sclerotiorum causes white mold, leading to substantial losses on a wide variety of hosts around the world. Many genes encoding effector proteins play important roles in the pathogenesis of S. sclerotiorum. Therefore, establishment of a transformation system for the exploration of gene function is necessarily significant. Here, we introduce a modified protocol to acquire protoplasts for transformation and generate knockout strains, which completements the transformation system of S. sclerotiorum.
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页数：8
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