Novel monoclonal antibody-based sensitive enzyme-linked immunosorbent assay and rapid immunochromatographic strip for sensitive detecting aristolochic acid I in herbal remedies

被引：3
|
作者：
Huang, Chang-Lun [1 ]
Wu, Shih-Wei [2 ]
Hsu, Tsai-Ching [3 ]
Yang, Chen-Yu [4 ]
Chung, Wei-Heng [4 ]
Lin, Xin-Jie [4 ]
Liu, Biing-Hui [2 ,6 ]
Yu, Feng-Yih [4 ,5 ,6 ]
机构：
[1] Changhua Christian Hosp, Dept Surg, Div Thorac Surg, Changhua, Taiwan
[2] Natl Taiwan Univ, Grad Inst Toxicol, Coll Med, Taipei, Taiwan
[3] Chung Shan Med Univ, Inst Med, Taichung, Taiwan
[4] Chung Shan Med Univ, Dept Biomed Sci, Taichung, Taiwan
[5] Chung Shan Med Univ Hosp, Dept Med Res, Taichung, Taiwan
[6] 110,Sect 1,Chien Kuo N Rd, Taichung 110, Taiwan
来源：
MICROCHEMICAL JOURNAL | 2023年 / 191卷
关键词：
Aristolochic acid; Monoclonal antibody; ELISA; Gold nanoparticle immunochromatographic; strip; HPLC;
D O I：
10.1016/j.microc.2023.108884
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Monoclonal antibodies (mAbs) specific to aristolochic acid I (AAI) were produced from a hybridoma cell line, 10D9E10, and used to establish a rapid and sensitive indirect enzyme-linked immunosorbent assay (ciELISA) and immunochromatographic strip (immunostrip) for detecting AAI in herbal remedies. In the ciELISA, the con-centration that caused 50% inhibition of the binding of antibodies to the solid-phase AAI-ovalbumin (OVA) by free AAI (IC50) and the detection limit (IC10) were calculated to be 0.4 and 0.03 ng/mL, respectively. The rapid on-site detection of immunostrip had a detection limit of 1 ng/mL for AAI. Analysis of 24 herbal remedy samples using the ciELISA showed AAI contamination levels ranging from 61 ng/g to 319 mu g/g. High-performance liquid chromatography (HPLC) analysis confirmed results obtained from ciELISA and immunostrip, supporting rapid on-site AAI detection in herbal remedies.
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页数：10
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