Improving the COI DNA barcoding library for Neotropical phlebotomine sand flies (Diptera: Psychodidae)

被引:4
|
作者
Posada-Lopez, Laura [1 ,2 ]
Rodrigues, Bruno Leite [2 ]
Velez, Ivan Dario [1 ]
Uribe, Sandra [3 ]
机构
[1] Univ Antioquia, PECET Programa Estudio & Control Enfermedades Trop, Medellin, Colombia
[2] Fac Saude Publ FSP USP, Programa Posgrad Saude Publ, Sao Paulo, SP, Brazil
[3] Univ Nacl Colombia, Grp Invest Sistemat Mol, Medellin, Colombia
关键词
Phlebotominae; Barcoding; Single-locus; Species delimitation; Molecular taxonomy; Molecular systematics; SPECIES DELIMITATION; SANDFLIES DIPTERA; IDENTIFICATION; TOOL; DIVERSITY; TCS;
D O I
10.1186/s13071-023-05807-z
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Sand fly species are traditionally identified using morphological traits, though this method is hampered by the presence of cryptic species. DNA barcoding is a widely used tool in the case of insects of medical importance, where it is necessary to know quickly which species are present in a transmission area. Here, we assess the usefulness of mitochondrial cytochrome c oxidase subunit I (COI) DNA barcoding as a practical tool for species identification, correct assignment of isomorphic females, and to evaluate the detection of cryptic diversity that occurs in the same species. A fragment of the COI gene was used to generate 156 new barcode sequences for sand flies from different countries of the Neotropical region, mainly Colombia, which had been identified morphologically as 43 species. The sequencing of the COI gene allowed the detection of cryptic diversity within species and correctly associated isomorphic females with males identified by morphology. The maximum intraspecific genetic distances ranged from 0 to 8.32% and 0 to 8.92% using uncorrected p distances and the Kimura 2-parameter (K2P) model, respectively. The minimum interspecific distance (nearest neighbor) for each species ranged from 1.5 to 14.14% and 1.51 to 15.7% using p and K2P distances, respectively. Three species had more than 3% maximum intraspecific distance: Psychodopygus panamensis, Micropygomyia cayennensis cayennensis, and Pintomyia evansi. They also were split into at least two molecular operational taxonomic units (MOTUs) each, using different species delimitation algorithms. Regarding interspecific genetic distances, the species of the genera Nyssomyia and Trichophoromyia generated values lower than 3% (except Nyssomyia ylephiletor and Ny. trapidoi). However, the maximum intraspecific distances did not exceed these values, indicating the presence of a barcode gap despite their proximity. Also, nine sand fly species were DNA barcoded for the first time: Evandromyia georgii, Lutzomyia sherlocki, Ny. ylephiletor, Ny. yuilli pajoti, Psathyromyia punctigeniculata, Sciopemyia preclara, Trichopygomyia triramula, Trichophoromyia howardi, and Th. velezbernali. The COI DNA barcode analysis enabled the correct delimitation of several Neotropical sand fly species from South and Central America and raised questions about the presence of cryptic species for some taxa, which should be further assessed.
引用
收藏
页数:12
相关论文
共 50 条
  • [41] PSYCHODOPYGUS AS A GENUS - REASONS FOR A FLEXIBLE CLASSIFICATION OF THE PHLEBOTOMINE SAND FLIES (DIPTERA, PSYCHODIDAE)
    READY, PD
    FRAIHA, H
    LAINSON, R
    SHAW, JJ
    JOURNAL OF MEDICAL ENTOMOLOGY, 1980, 17 (01) : 75 - 88
  • [42] Morphological keys for the identification of Italian phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae)
    Filipe Dantas-Torres
    Viviana Domenica Tarallo
    Domenico Otranto
    Parasites & Vectors, 7
  • [43] LABORATORY EVALUATION OF CHLORFLUAZURON AGAINST LARVAL PHLEBOTOMINE SAND FLIES (DIPTERA, PSYCHODIDAE)
    QUESADA, BL
    MONTOYALERMA, J
    JOURNAL OF ECONOMIC ENTOMOLOGY, 1994, 87 (05) : 1129 - 1132
  • [44] Phlebotomine sand flies (Diptera: Psychodidae) in the Greek Aegean Islands: ecological approaches
    Tsirigotakis, Nikolaos
    Pavlou, Christoforos
    Christodoulou, Vasiliki
    Dokianakis, Emmanouil
    Kourouniotis, Christos
    Alten, Bulent
    Antoniou, Maria
    PARASITES & VECTORS, 2018, 11
  • [45] An updated checklist of the phlebotomine sand flies of Veracruz, Mexico (Diptera: Psychodidae, Phlebotominae)
    Ibanez-Bernal, Sergio
    Suarez-Landa, Teresa
    Mendoza, Fredy
    ZOOTAXA, 2011, (2928) : 29 - 40
  • [46] Phlebotomine Sand Flies (Diptera: Psychodidae: Phlebotominae) of Alagoas State, Northeast of Brazil
    Andrade Filho, Jose D.
    Brazil, Reginaldo P.
    NEOTROPICAL ENTOMOLOGY, 2009, 38 (05) : 688 - 690
  • [47] Phlebotomine sand flies (Diptera: Psychodidae) and their epidemiological importance. Literature review
    Tanczos Balazs
    Farkas Robert
    MAGYAR ALLATORVOSOK LAPJA, 2009, 131 (07) : 411 - 416
  • [48] Phlebotomine sand flies (Diptera: Psychodidae) in the Greek Aegean Islands: ecological approaches
    Nikolaos Tsirigotakis
    Christoforos Pavlou
    Vasiliki Christodoulou
    Emmanouil Dokianakis
    Christos Kourouniotis
    Bulent Alten
    Maria Antoniou
    Parasites & Vectors, 11
  • [49] NEW SPECIES AND RECORDS OF PHLEBOTOMINE SAND FLIES FROM COLOMBIA (DIPTERA, PSYCHODIDAE)
    YOUNG, DG
    MORALES, A
    JOURNAL OF MEDICAL ENTOMOLOGY, 1987, 24 (06) : 651 - 665
  • [50] DNA barcoding to identify species of phlebotomine sand fly (Diptera: Psychodidae) in the mixed leishmaniasis focus of the Colombian Caribbean
    Romero-Ricardo, Luis
    Lastre-Meza, Natalia
    Perez-Doria, Alveiro
    Bejarano, Eduar E.
    ACTA TROPICA, 2016, 159 : 125 - 131