Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/ Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice

被引：2
|
作者：
Gao, Hongyan [1 ]
Li, Yanhui [2 ]
Jin, Yue [1 ]
Zhang, Li [1 ]
Xia, Xiuwen [1 ]
Liu, Jinkun [2 ]
Wang, Huaifu [1 ]
Xie, Ya [3 ]
Ding, Weijun [1 ,4 ]
机构：
[1] Chengdu Univ Tradit Chinese Med, Sch Basic Med Sci, Chengdu 611137, Peoples R China
[2] Chongqing Hosp Tradit Chinese Med, Chongqing 400021, Peoples R China
[3] Jinniu Hosp, Sichuan Prov Peoples Hosp, Chengdu 610007, Peoples R China
[4] Chengdu Univ Tradit Chinese Med, Sch Basic Med Sci, 1166 Liutai Ave, Chengdu 611137, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2023年 / 166卷
基金：
中国国家自然科学基金;
关键词：
Electroacupuncture (EA); White adipose tissue (WAT); Browning; Angiogenesis; Obesity; DIET-INDUCED OBESITY; VEGF-A; INSULIN-RESISTANCE; THROMBOSPONDIN-1; INFLAMMATION; ACUPUNCTURE; FAT; OVEREXPRESSION; DYSFUNCTION; CELL;
D O I：
10.1016/j.biopha.2023.115386
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
This study investigated the effect of electroacupuncture (EA) on the browning of white adipose tissue (WAT) via angiogenesis and its potential mechanism in obese mice. Four-week-old male C56BL/6 mice were randomly divided into a high-fat diet (HFD) and a normal chow diet (ND) group. After 12 weeks, HFD mice were randomly divided into two groups to receive or not receive EA for 3 weeks. After EA treatment, body weight, adipocyte size, serum glucose (GLU), triacylglycerol (TG), cholesterol (CHO), leptin (Lep), monocyte chemoattractant protein-1 (MCP-1), WAT browning-related genes, angiogenesis-related genes, and the PI3K/Pten/Thbs1 signaling pathway were evaluated. The results indicated that EA significantly reduced body weight, adipocyte size, and serum concentrations of GLU, TG, CHO, Lep and MCP-1 and promoted WAT browning. Angiogenesis and the PI3K/Pten/Thbs1 signaling pathway were all activated by EA intervention. The expression levels were consistent with the results of RNA-seq and confirmed via qRT-PCR and WB. Our study showed that EA may activate angiogenesis via the PI3K/Pten/Thbs1 signaling pathway in WAT, thereby promoting the browning and ther-mogenesis of adipose tissue.
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页数：11
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