Taking a step back from testing: Preanalytical considerations in molecular infectious disease diagnostics

被引：3

作者：

Conrad, Stephanie ^{[1
]}

Kanegusuku, Anastasia Gant ^{[2
]}

Conklin, Steven E. ^{[1
,3
]}

机构：

[1] Tufts Med Ctr, Dept Pathol & Lab Med, Boston, MA 19716 USA

[2] Univ Chicago, Dept Pathol, Chicago, IL USA

[3] Tufts Univ, Dept Anat & Clin Pathol, Sch Med, Boston, MA 19716 USA

来源：

CLINICAL BIOCHEMISTRY | 2023年 / 115卷

关键词：

Preanalytics; Molecular diagnostics; Infectious disease; POLYMERASE CHAIN-REACTION; NUCLEIC-ACID; ANTIRETROVIRAL THERAPY; TRANSPORT MEDIA; PCR INHIBITORS; WHOLE-BLOOD; STORAGE; STABILITY; DNA; SPECIMEN;

D O I：

10.1016/j.clinbiochem.2022.12.003

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Recent studies evaluating the preanalytical factors that impact the outcome of nucleic-acid based methods for the confirmation of SARS-CoV-2 have illuminated the importance of identifying variables that promoted accurate testing, while using scarce resources efficiently. The majority of laboratory errors occur in the preanalytical phase. While there are many resources identifying and describing mechanisms for main laboratory testing on automated platforms, there are fewer comprehensive resources for understanding important preanalytical and environmental factors that affect accurate molecular diagnostic testing of infectious diseases. This review identifies evidence-based factors that have been documented to impact the outcome of nucleic acid-based molecular techniques for the diagnosis of infectious diseases.

引用

页码：22 / 32

页数：11

共 50 条

[31] Molecular characterization of an Infectious bursal disease virus isolate from Iran
Hosseini, SD
Omar, AR
Aini, I
ACTA VIROLOGICA, 2004, 48 (02) : 79 - 83
[32] Taking a step back for a leap forward: policy formation for the digitalisation of schools from the views of Swedish national policymakers
Gustafsson, Ulrika
EDUCATION INQUIRY, 2021, 12 (04) : 329 - 346
[33] Testing for equine infectious anemia (Reprinted from Equine Disease Quarterly, vol 7)
不详
EQUINE PRACTICE, 1999, 21 (06): : 13 - 13
[34] SEROLOGICAL TESTING IN A MICROBIOLOGY LABORATORY OF SPECIMENS FROM PATIENTS WITH SUSPECTED INFECTIOUS-DISEASE
WAGHORN, DJ
JOURNAL OF CLINICAL PATHOLOGY, 1995, 48 (04) : 358 - 363
[35] EFFECT OF DRAWING SAMPLES FOR INFECTIOUS-DISEASE TESTING FROM TUBING OF THE PRIMARY CONTAINER
AUBUCHON, JP
FANG, C
MCCURDY, K
ZIMMERMAN, B
FOREY, JE
COBURN, TJ
TRANSFUSION, 1987, 27 (06) : 524 - 524
[36] Molecular characterization of field infectious bursal disease virus isolates from Nigeria
Nwagbo, Ijeoma O.
Shittu, Ismaila
Nwosuh, Chika I.
Ezeifeka, George O.
Odibo, Frederick J. C.
Michel, Linda O.
Jackwood, Daral J.
VETERINARY WORLD, 2016, 9 (12) : 1420 - 1428
[37] Molecular characterization of recent infectious bursal disease virus isolates from Malaysia
Nurulfiza, I.
Hair-Bejo, M.
Omar, A. R.
Aini, I.
ACTA VIROLOGICA, 2006, 50 (01) : 45 - 51
[38] Real-life impact on antimicrobial prescription of Syndromic Molecular Testing in adults hospitalized in infectious disease departments. Respiratory Syndromic Molecular Testing panel: Is it worth it?
Clauss, Severine
Bourlet, Stephane
Jaffal, Karim
Duran, Clara
D'Anglejan, Emma
Perronne, Veronique
Bouchand, Frederique
Noussair, Latifa
Dahmane, Lotfi
Dinh, Aurelien
INFECTIOUS DISEASES NOW, 2024, 54 (05):
[39] New Foci for the Genetic Testing Reference Material (GeT-RM) Program: Molecular Oncology and Infectious Disease
Barker, S.
Bossler, A. D.
Schneider, E.
Kalman, L.
JOURNAL OF MOLECULAR DIAGNOSTICS, 2008, 10 (06): : 597 - 597
[40] DNA repair and neurological disease: From molecular understanding to the development of diagnostics and model organisms
Abugable, Arwa A.
Morris, Julia L. M.
Palminha, Nelma M.
Zaksauskaite, Ringaile
Ray, Swagat
E-Khamisy, Sherif F.
DNA REPAIR, 2019, 81

← 1 2 3 4 5 →