Method for extracting nematode cysts of the family Heteroderidae from sandy loam soil

被引：1
|
作者：
da Silva, Francisco Bruno [1 ]
Carmem, Cafe [1 ]
Santos, Dolores Gonzaga [1 ]
Dolores, Carmem [1 ]
Santos, Gonzaga [1 ]
机构：
[1] Univ Fed Ceara, Dept Fitotecnia, Campus Pici, BR-60356001 Fortaleza, Ceara, Brazil
来源：
AUSTRALASIAN PLANT PATHOLOGY | 2023年 / 52卷 / 01期
关键词：
Extraction technique; Soil sample processing; Phytonematodes;
D O I：
10.1007/s13313-022-00861-0
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The method of extracting nematodes from the soil developed by Jenkins was combined with the Coolen and D'Herde technique with the aim of optimising the extraction of heteroderid cysts from the soil. In addition to the soil, the combined methodology employs 40-mesh (420 mu m) and 200-mesh (74 mu m) sieves, kaolin and sucrose. Using this method, it is possible to obtain quickly almost four times the number of cysts in an almost clear suspension, compared to the traditional method of cyst-extraction devised by Shepherd.
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页码：43 / 44
页数：2
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