Evaluation of Antibiofilm and Antiquorum Sensing Activities of Fucoidan Characterized from Padina boryana against Nosocomial Pathogens

Modern functional chemicals that can be employed in biotechnology, pharmaceutics, and food science are a sustainable source to be found in seaweeds. The bioactivity of the majority of these marine compounds has received scant research. Fucoidan is a highly sulfated polysaccharide with a range of bioactivities, including an antipathogenic effect. There is still much to learn about the relationship between fucoidan structure and its function in pathogen infections. By employing microwave and probe sonication to create crude fucoidan, DEAE-cellulose anion-exchange chromatography was used to further purify the substance. Purified fucoidan was structurally characterized using UV-Visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and NMR analysis. The results of the structural analysis demonstrate that sulfates and hydroxyl groups are present in the isolated fucoidan. There are fucose residues, according to the NMR data. The present study investigates the bioactivity of fucoidan, a polysaccharide derived from the brown algae Padina boryana, as a potent weapon against the known nosocomial diseases Proteus vulgaris and Salmonella enterica. Fluorescence microscopy was used to show that fucoidan antibiofilm action is totally effective against Proteus vulgaris and Salmonella enterica biofilm formations as well as planktonic cell growths at dosages over 25 g/mL. Here, using in vitro investigations of the possible inactivation of molecules that are regulated by acyl-homoserine lactone (AHL) in both bacterial species, we explore the antiquarum sensing and antibiofilm capabilities of fucoidan. According to the present study, extracted fucoidan from Padina boryana can be used to generate antibacterial compounds and operate as a quorum-sensing inhibitor to combat side effects and antibiotic resistance.