The Evolution of Post-Vaccine G8P[4] Group a Rotavirus Strains in Rwanda; Notable Variance at the Neutralization Epitope Sites

被引:0
|
作者
Mwangi, Peter N. [1 ]
Potgieter, Robyn-Lee [1 ]
Uwimana, Jeannine [2 ]
Mutesa, Leon [2 ,3 ]
Muganga, Narcisse [2 ]
Murenzi, Didier [2 ]
Tusiyenge, Lisine [2 ]
Mwenda, Jason M. [4 ]
Mogotsi, Milton T. [1 ]
Rakau, Kebareng [5 ]
Esona, Mathew D. [5 ]
Steele, A. Duncan [5 ]
Seheri, Mapaseka L. [5 ]
Nyaga, Martin M. [1 ]
机构
[1] Univ Free State, Fac Hlth Sci, Div Virol, Next Generat Sequencing Unit, ZA-9300 Bloemfontein, South Africa
[2] Univ Rwanda, Kigali Univ, Teaching Hosp, Coll Med & Hlth Sci, POB 4285, Kigali, Rwanda
[3] Univ Rwanda, Coll Med & Hlth Sci, Ctr Human Genet, POB 4285, Kigali, Rwanda
[4] World Hlth Org, Reg Off Africa, POB 06, Brazzaville, Rep Congo
[5] Sefako Makgatho Hlth Sci Univ MEDUNSA, Diarrhoeal Pathogens Res Unit, ZA-0204 Pretoria, South Africa
来源
PATHOGENS | 2023年 / 12卷 / 05期
基金
新加坡国家研究基金会;
关键词
G8P[4; rotavirus; Rwanda; whole-genome sequencing; rotavirus reassortment; MOLECULAR CHARACTERIZATION; GENOMIC CONSTELLATION; BOVINE ROTAVIRUSES; SEVERE DIARRHEA; G8; STRAINS; VP7; GENE; INFANTS; GENOTYPE; GASTROENTERITIS; CHILDREN;
D O I
10.3390/pathogens12050658
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Africa has a high level of genetic diversity of rotavirus strains, which is suggested to be a possible reason contributing to the suboptimal effectiveness of rotavirus vaccines in this region. One strain that contributes to this rotavirus diversity in Africa is the G8P[4]. This study aimed to elucidate the entire genome and evolution of Rwandan G8P[4] strains. Illumina sequencing was performed for twenty-one Rwandan G8P[4] rotavirus strains. Twenty of the Rwandan G8P[4] strains had a pure DS-1-like genotype constellation, and one strain had a reassortant genotype constellation. Notable radical amino acid differences were observed at the neutralization sites when compared with cognate regions in vaccine strains potentially playing a role in neutralization escape. Phylogenetic analysis revealed that the closest relationship was with East African human group A rotavirus (RVA) strains for five of the genome segments. Two genome sequences of the NSP4 genome segment were closely related to bovine members of the DS-1-like family. Fourteen VP1 and eleven VP3 sequences had the closest relationships with the RotaTeq (TM) vaccine WC3 bovine genes. These findings suggest that the evolution of VP1 and VP3 might have resulted from reassortment events with RotaTeq (TM) vaccine WC3 bovine genes. The close phylogenetic relationship with East African G8P[4] strains from Kenya and Uganda suggests co-circulation in these countries. These findings highlight the need for continued whole-genomic surveillance to elucidate the evolution of G8P[4] strains, especially after the introduction of rotavirus vaccination.
引用
收藏
页数:19
相关论文
共 29 条
  • [1] Genomic characterization of uncommon human G3P [6] rotavirus strains that have emerged in Kenya after rotavirus vaccine introduction, and pre-vaccine human G8P [4] rotavirus strains
    Wandera, Ernest Apondi
    Komoto, Satoshi
    Mohammad, Shah
    Ide, Tomihiko
    Bundi, Martin
    Nyangao, James
    Kathiiko, Cyrus
    Odoyo, Erick
    Galata, Amina
    Miring'u, Gabriel
    Fukuda, Saori
    Hatazawa, Riona
    Murata, Takayuki
    Taniguchi, Koki
    Ichinose, Yoshio
    [J]. INFECTION GENETICS AND EVOLUTION, 2019, 68 : 231 - 248
  • [2] Emergence of unusual rotavirus G9P[4] and G8P[8] strains during post vaccination surveillance in Argentina, 2017-2018
    Degiuseppe, Juan Ignacio
    Andr, Juan
    [J]. INFECTION GENETICS AND EVOLUTION, 2021, 93
  • [3] Evolution of human G4P[8] group A rotavirus strains circulating in Italy in 2013
    Ianiro, Giovanni
    Delogu, Roberto
    Fiore, Lucia
    Ruggeri, Franco M.
    [J]. VIRUS RESEARCH, 2015, 204 : 68 - 73
  • [4] Characterization of an Unusual DS-1-Like G8P[8] Rotavirus Strain from Japan in 2017: Evolution of Emerging DS-1-Like G8P[8] Strains through Reassortment
    Kamiya, Hajime
    Tacharoenmuang, Ratana
    Ide, Tomihiko
    Negoro, Manami
    Tanaka, Takaaki
    Asad, Kazutoyo
    Nakamur, Haruna
    Sugiur, Katsumi
    Umemoto, Masakazu
    Kuroki, Haruo
    Ito, Hiroaki
    Tanaka, Shigeki
    It, Mitsue
    Fukuda, Saori
    Hatazawa, Riona
    Hara, Yuya
    Guntapong, Ratigorn
    Murata, Takayuki
    Taniguchi, Kiyosu
    Suga, Shigeru
    Nakano, Takashi
    Taniguchi, Koki
    Komoto, Satoshi
    [J]. JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2019, 72 (04) : 256 - 260
  • [5] Molecular Epidemiology of Rotavirus A Strains Pre- and Post-Vaccine (Rotarix(R)) Introduction in Mozambique, 2012-2019: Emergence of Genotypes G3P[4] and G3P[8]
    Joao, Eva D.
    Munlela, Benilde
    Chissaque, Assucenio
    Chilaule, Jorfelia
    Langa, Jeronimo
    Augusto, Orvalho
    Boene, Simone S.
    Anapakala, Elda
    Sambo, Julia
    Guimaraes, Esperanca
    Bero, Diocreciano
    Cassocera, Marta
    Cossa-Moiane, Idalecia
    Mwenda, Jason M.
    Mauricio, Isabel
    O'Neill, Hester G.
    de Deus, Nilsa
    [J]. PATHOGENS, 2020, 9 (09): : 1 - 16
  • [6] Molecular characterization of unusual bovine group A rotavirus G8P[14] strains identified in western India: Emergence of P[14] genotype
    Chitambar, Shobha D.
    Arora, Ritu
    Kolpe, Annasaheb B.
    Yadav, Mahendra M.
    Raut, Chadrashekhar G.
    [J]. VETERINARY MICROBIOLOGY, 2011, 148 (2-4) : 384 - 388
  • [7] Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012-2017)
    Munlela, Benilde
    Joao, Eva D.
    Donato, Celeste M.
    Strydom, Amy
    Boene, Simone S.
    Chissaque, Assucenio
    Bauhofer, Adilson F. L.
    Langa, Jeronimo
    Cassocera, Marta
    Cossa-Moiane, Idalecia
    Chilaule, Jorfelia J.
    O'Neill, Hester G.
    de Deus, Nilsa
    [J]. PATHOGENS, 2020, 9 (12): : 1 - 19
  • [8] Molecular characterization of human G8P[4] rotavirus strains in Italy: Proposal of a more complete subclassification of the G8 genotype in three major lineages
    Ianiro, Giovanni
    Delogu, R.
    Bonomo, P.
    Castiglia, P.
    Ruggeri, F. M.
    Fiore, L.
    [J]. INFECTION GENETICS AND EVOLUTION, 2014, 21 : 129 - 133
  • [9] Genomic epidemiology of the rotavirus G2P[4] strains in coastal Kenya pre- and post-rotavirus vaccine introduction, 2012-8
    Makori, Timothy O.
    Bargul, Joel L.
    Lambisia, Arnold W.
    Mwanga, Mike J.
    Murunga, Nickson
    de Laurent, Zaydah R.
    Lewa, Clement S.
    Mutunga, Martin
    Kellam, Paul
    Cotten, Matthew
    Nokes, D. James
    Phan, My
    Agoti, Charles N.
    [J]. VIRUS EVOLUTION, 2023, 9 (01)
  • [10] Whole-genome analyses of DS-1-like human G2P[4] and G8P[4] rotavirus strains from Eastern, Western and Southern Africa
    Nyaga, Martin M.
    Stucker, Karla M.
    Esona, Mathew D.
    Jere, Khuzwayo C.
    Mwinyi, Bakari
    Shonhai, Annie
    Tsolenyanu, Enyonam
    Mulindwa, Augustine
    Chibumbya, Julia N.
    Adolfine, Hokororo
    Halpin, Rebecca A.
    Roy, Sunando
    Stockwell, Timothy B.
    Berejena, Chipo
    Seheri, Mapaseka L.
    Mwenda, Jason M.
    Steele, A. Duncan
    Wentworth, David E.
    Mphahlele, M. Jeffrey
    [J]. VIRUS GENES, 2014, 49 (02) : 196 - 207