Fluorescent Properties of Cyanine Dyes As a Matter of the Environment

被引：4

作者：

Fan, Fan ^{[1
]}

Povedailo, Vladimir A. ^{[2
]}

Lysenko, Ivan L. ^{[1
]}

Seviarynchyk, Tatsiana P. ^{[1
]}

Sharko, Olga L. ^{[1
]}

Mazunin, Ilya O. ^{[3
]}

Shmanai, Vadim V. ^{[1
]}

机构：

[1] Natl Acad Sci Belarus, Inst Phys Organ Chem, 13 Surganova str, Minsk 220072, BELARUS

[2] Natl Acad Sci Belarus, BI Stepanov Inst Phys, 68 Nezavisimost Ave, Minsk 220072, BELARUS

[3] Skolkovo Inst Sci & Technol, Ctr Mol & Cellular Biol, Moscow, Russia

来源：

JOURNAL OF FLUORESCENCE | 2024年 / 34卷 / 02期

基金：

俄罗斯科学基金会;

关键词：

Fluorescence intensity; Fluorescence lifetime; Fluorescence quantum yields; Cyanine dyes; Click chemistry; DOUBLE-STRANDED DNA; SYNTHETIC APPROACH; TEMPERATURE-DEPENDENCE; PHOTOISOMERIZATION; ISOMERIZATION; CARBOCYANINES; PHOTOPHYSICS; ENHANCEMENT; VISCOSITY; DYNAMICS;

D O I：

10.1007/s10895-023-03321-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In non-viscous aqueous solutions, the cyanine fluorescent dyes Cy3 and Cy5 have rather low fluorescence efficiency (the fluorescence quantum yields of Cy3 and Cy5 are 0.04 and 0.3, respectively [1, 2]) and short excited state lifetimes due to their structural features. In this work, we investigated the effect of solubility and rotational degrees of freedom on the fluorescence efficiency of Cy3 and Cy5 in several ways. We compared the fluorescence efficiencies of two cyanine dyes sCy3 and sCy5 with the introduction of a sulfonyl substituent in the aromatic ring as well as covalently bound to T10 oligonucleotides. The results show that because of the different lengths of the polymethine chains between the aromatic rings of the dyes, cis-trans-isomerization has a much greater effect on the Cy3 molecule than on the Cy5 molecule, while the effect of aggregation is also significant.

引用

页码：925 / 933

页数：9

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