Enzyme-bimetallic hybrid catalyst for one-pot chemoenzymatic reactions

To overcome the intrinsic incompatibility between enzyme and chemical catalysts in one-pot chemoenzymatic cascade reactions, an enzyme-bimetallic hybrid catalyst was constructed by in situ reduction of ultrafine PdCu naonoclusters on the cross-linked lipase aggregates. The PdCu nanoalloy showed excellent activity in the Sonogashira coupling reaction because of the strong bimetallic synergistic effect between Pd and Cu within PdCu/CALB CLEAs. The activity of 3.4Pd1.7Cu/CALB CLEAs was 2.6-fold of Pd/CALB CLEAs in the synthesis of (R)-N-[1-(4-(phenylethynyl)phenyl)ethyl] acetamide. The highly active PdCu/CALB CLEAs rendered the one-pot chemoenzymatic reaction to perform at a lower temperature.