Combination Analysis of PCDHGA12 and CDO1 DNA Methylation in Bronchial Washing Fluid for Lung Cancer

被引:3
|
作者
Park, Se Jin [1 ]
Kang, Daeun [1 ]
Lee, Minhyeok [1 ]
Lee, Su Yel [2 ]
Park, Young Gyu [3 ]
Oh, TaeJeong [4 ]
Jang, Seunghyun [4 ]
Hwang, Wan Jin [5 ]
Kwon, Sun Jung [1 ]
An, Sungwhan [4 ]
Son, Ji Woong [1 ]
Jeong, In Beom [1 ]
机构
[1] Konyang Univ Hosp, Dept Internal Med, Div Pulmonol, 158 Gwanjeodong Ro, Daejeon 35365, South Korea
[2] Konyang Univ, Myunggok Med Res Inst, Coll Med, Daejeon, South Korea
[3] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Oncol, Seoul, South Korea
[4] Genomictree Inc, Daejeon, South Korea
[5] Konyang Univ Hosp, Dept Thorac & Cardiovasc Surg, Daejeon, South Korea
基金
新加坡国家研究基金会;
关键词
Lung Cancer; DNA Methylation; PCDHGA12; CDO1; Bronchial Washing Fluid; PROMOTER HYPERMETHYLATION; BRONCHOALVEOLAR LAVAGE; DIAGNOSIS; GENES; EPIGENETICS; BIOMARKERS; SURVIVAL; ASPIRATE; RASSF1A; BIOPSY;
D O I
10.3346/jkms.2024.39.e28
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: When suspicious lesions are observed on computer-tomography (CT), invasive tests are needed to confirm lung cancer. Compared with other procedures, bronchoscopy has fewer complications. However, the sensitivity of peripheral lesion through bronchoscopy including washing cytology is low. A new test with higher sensitivity through bronchoscopy is needed. In our previous study, DNA methylation of PCDHGA12 in bronchial washing cytology has a diagnostic value for lung cancer. In this study, combination of PCDHGA12 and CDO1 methylation obtained through bronchial washing cytology was evaluated as a diagnostic tool for lung cancer. Methods: A total of 187 patients who had suspicious lesions in CT were enrolled. PCDHGA12 methylation test, CDO1 methylation test, and cytological examination were performed using 3-plex LTE-qMSP test. Results: Sixty-two patients were diagnosed with benign diseases and 125 patients were diagnosed with lung cancer. The sensitivity of PCDHGA12 was 74.4% and the specificity of PCDHGA12 was 91.9% respectively. CDO1 methylation test had a sensitivity of 57.6% and a specificity of 96.8%. The combination of both PCDHGA12 methylation test and CDO1 methylation test showed a sensitivity of 77.6% and a specificity of 90.3%. The sensitivity of lung cancer diagnosis was increased by combining both PCDHGA12 and CDO1 methylation tests. Conclusion: Checking DNA methylation of both PCDHGA12 and CDO1 genes using bronchial washing fluid can reduce the invasive procedure to diagnose lung cancer.
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页数:11
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