Human cytokeratin 1 plays a role in the interaction of Pteropine orthoreovirus with Hek293 cells but not HeLa cells

被引：1

作者：

Siew, Zhen Yun ^{[1
]}

Tan, Yeh Fong ^{[2
,3
,4
,5
]}

Iswara, Reuben Parama ^{[2
]}

Wong, Shew Fung ^{[2
,6
]}

Wong, Siew Tung ^{[2
]}

Tan, Boon Keat ^{[2
]}

Leong, Pooi Pooi ^{[7
]}

Tan, Chee Wah ^{[8
,10
]}

Wang, Lin -Fa ^{[8
]}

Leong, Chee Onn ^{[6
,9
]}

Voon, Kenny ^{[1
,2
]}

机构：

[1] Univ Nottingham Malaysia, Sch Pharm, Semenyih, Malaysia

[2] Int Med Univ, Sch Med, Kuala Lumpur, Malaysia

[3] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan

[4] Natl Yang Ming Chiao Tung Univ, Taiwan Int Grad Program Mol Med, Taipei, Taiwan

[5] Acad Sinica, Taipei, Taiwan

[6] Int Med Univ, Inst Res Dev & Innovat IRDI, Kuala Lumpur, Malaysia

[7] Univ Tunku Abdul Rahman, Fac Med & Hlth Sci, Sg Long, Malaysia

[8] Duke NUS Med Sch, Programme Emerging Infect Dis, Singapore 169857, Singapore

[9] AGTC Genom, Kuala Lumpur 57000, Malaysia

[10] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol & Immunol, Infect Dis Translat Res Programme, Singapore, Singapore

来源：

MICROBES AND INFECTION | 2024年 / 26卷 / 1-2期

关键词：

Pulau virus; Kampar virus; Human cytokeratin 1; Cellular receptor; Zoonotic virus; Cell attachment; Pteropine orthoreovirus (PRV); ASSAY;

D O I：

10.1016/j.micinf.2023.105243

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pteropine orthoreovirus (PRV) causes respiratory tract infections in humans. Despite its emergence as a zoonotic and respiratory virus, little is known about its cell tropism, which hampers progress in fully understanding its pathogenesis in humans. Hek293 cells are most susceptible to PRV infection, while HeLa cells are the least. Human cytokeratin 1 (CK1) was identified as the protein that interacts with PRV. The immunofluorescence assay and qPCR results revealed prior treatment with anti-CK1 may provide Hek293 cells protection against PRV. The KRT1-knockout Hek293 cells were less susceptible to PRV infection. Further study into the pathogenesis of PRV in humans is needed. (c) 2023 The Author(s). Published by Elsevier Masson SAS on behalf of Institut Pasteur. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

引用

页数：5

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