Adamantoyl chloride inhibited replication of the largemouth bass virus via enhanced immunity and inhibition of apoptosis

被引:4
|
作者
Wang, Mengmeng [1 ]
Yang, Bin [1 ]
Liu, Tao [1 ]
Li, Pengfei [2 ]
Bai, Shangjie [1 ]
Zhou, Zhengyang [1 ]
Liu, Xiang [1 ]
He, Maosheng [1 ]
Ling, Fei [1 ]
Wang, Gaoxue [1 ,3 ]
机构
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
[2] Guangxi Acad Sci, Guangxi Key Lab Aquat Biotechnol & Modern Ecol Aqu, Nanning, Peoples R China
[3] Northwest A&F Univ, Xinong Rd 22nd, Yangling 712100, Shaanxi, Peoples R China
关键词
Adamantoyl chloride; Largemouth bass virus; Antiviral effect; Innate immunity; EFFICACY; ANTIVIRALS; VACCINE;
D O I
10.1016/j.fsi.2023.109167
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The largemouth bass virus (LMBV) is a commonly encountered pathogen in aquaculture and presents significant challenges to development of the largemouth bass industry due to the lack of effective treatment methods. Here, the inhibitory potential and underlying mechanisms of adamantoyl chloride (AdCl) against LMBV were assessed both in vitro and in vivo. The results showed that AdCl (IC50 = 72.35 mu M) significantly inhibited replication of LMBV in epithelioma papulosum cyprini (EPC) cells. The results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl2H-tetrazolium bromide and cytopathic effect (CPE) assays confirmed that AdCl inhibited replication of LMBV in EPC cells and significantly reduced the CPE effect, respectively. As a potential mechanism, AdCl inhibited apoptosis as determined by fluorescence and transmission electron microscopy. The results of flow cytometry showed that the apoptosis rate was decreased by 69 % in the AdCl-treated group as compared to the LMBVinfected group. Additionally, AdCl inhibited viral release. In vivo, the survival rate was 16.2 % higher in the AdCl-treated group as compared to the LMBV-infected group (26.9 % vs. 10.7 %, respectively). Additionally, the results of quantitative reverse transcription polymerase chain reaction (RT-qPCR) showed that AdCl significantly reduced the viral load of the fish liver, spleen, and kidneys at 3, 6, and 9 days postinfection. In addition, RT-qPCR analysis found that AdCl upregulated expression of immune-related genes to suppress replication of LMBV. Collectively, these results confirmed the anti-LMBV activities of AdCl for use in the aquaculture industry.
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页数:11
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