Antimicrobial Potency of Green Synthesized Silver Nanoparticles from Stem Extract of Euphorbia poissoniion Urinary Tract Pathogens

Euphorbia poissonii is a medicinal plant containing bioactive compounds of therapeutic importance. However, few works using the green technology have been done on this plant as an antimicrobial agent against urinary tract pathogens. The antimicrobial potency of synthesized nanoparticles from Euphorbia poissonii was investigated against urinary tract pathogens collected from Dalhatu Araf Specialist Hospital, Lafia, Nigeria. Euphorbia poissonii silver nanoparticles (EP-AgNPs) was synthesized using the Euphorbia poissonii stem extract as the reducing and functionalizing agent. FTIR was used to determine the functional groups in the plant extract responsible for the reduction and the capping of the nanoparticles. The nanosilver was characterized by UV-Vis, scanning electron microscope (SEM), X-ray diffraction (XRD), transmission electron microscope (TEM) and energy dispersion X-ray analyzer (EDX). UV-visible analysis showed the absorption peak at 420 nm. TEM micrograph showed AgNPs of an average particle size of 10.25 nm. The EP-AgNPs were highly potent at 25-50 mg/L MIC than the crude stem extracts at 100-200 mg/L MIC, against Staphylococcus aureus, Candida albicans and Escherichia coli. A 50 mg/L MFC and 50 mg/mL- 100 mg/L MBC ofEP-AgNPs inactivated Candida albicans, E. coli and Klebsiella pneumonia, respectively. Data obtained shows an environmentally friendly greener approach for the production of future therapeutics against uinary tract pathogens. [GRAPHICS]