An intelligent smartphone-test strip detection platform for rapid and on-site sensing of benzoyl peroxide in flour samples

Benzoyl peroxide (BPO) is a commonly used flour whitener, but its excessive usage can have adverse effects on human health, such as nutrient loss, vitamin deficiencies and certain diseases. In this study, a europium metal organic framework (Eu-MOF) fluorescence probe was prepared, which exhibited a strong fluorescence emission at 614 nm upon excitation at 320 nm, with a high quantum yield of 8.11%. The red fluorescence of the probe could be effectively quenched by BPO through the inner filter effect (IFE) and photoinduced electron transfer (PET) mechanism. The detection process offered several advantages, including a wide linear range of 0-0.95 mM, a low detection limit of 66 nM and a fast fluorescence response of 2 min. Furthermore, an intelligent detection platform was designed to enhance the practical application of the detection method. This platform combined the portability and visuality of a traditional test strip with the color recognition capability of a smartphone, allowing for the visualization and quantitative detection of BPO in a convenient and user-friendly manner. The detection platform was successfully applied to the analysis of BPO in real flour samples with satisfactory recoveries (99.79%-103.94%), suggesting a promising strategy for the rapid and on-site detection of BPO in food samples.