Pseudomonas aeruginosa two-component system CprRS regulates HigBA expression and bacterial cytotoxicity in response to LL-37 stress
被引:4
|
作者:
Song, Yingjie
论文数: 0引用数: 0
h-index: 0
机构:
Sichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Song, Yingjie
[1
]
Zhang, Siping
论文数: 0引用数: 0
h-index: 0
机构:
Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Zhang, Siping
[2
]
Zhao, Ninglin
论文数: 0引用数: 0
h-index: 0
机构:
Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Zhao, Ninglin
[3
]
Nong, Cheng
论文数: 0引用数: 0
h-index: 0
机构:
Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Nong, Cheng
[3
]
He, Yongxing
论文数: 0引用数: 0
h-index: 0
机构:
Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
He, Yongxing
[2
]
Bao, Rui
论文数: 0引用数: 0
h-index: 0
机构:
Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Bao, Rui
[3
]
机构:
[1] Sichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
[2] Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou, Peoples R China
[3] Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu, Peoples R China
Pseudomonas aeruginosa is a highly pathogenic bacterium known for its ability to sense and coordinate the production of virulence factors in response to host immune responses. However, the regulatory mechanisms underlying this process have remained largely elusive. In this study, we investigate the two-component system CprRS in P. aeruginosa and unveil the crucial role of the sensor protein CprS in sensing the human host defense peptide LL-37, thereby modulating bacterial virulence. We demonstrate that CprS acts as a phosphatase in the presence of LL-37, leading to the phosphorylation and activation of the response regulator CprR. The results prove that CprR directly recognizes a specific sequence within the promoter region of the HigBA toxin-antitoxin system, resulting in enhanced expression of the toxin HigB. Importantly, LL-37-induced HigB expression promotes the production of type III secretion system effectors, leading to reduced expression of proinflammatory cytokines and increased cytotoxicity towards macrophages. Moreover, mutations in cprS or cprR significantly impair bacterial survival in both macrophage and insect infection models. This study uncovers the regulatory mechanism of the CprRS system, enabling P. aeruginosa to detect and respond to human innate immune responses while maintaining a balanced virulence gene expression profile. Additionally, this study provides new evidence and insights into the complex regulatory system of T3SS in P. aeruginosa within the host environment, contributing to a better understanding of host-microbe communication and the development of novel strategies to combat bacterial infections.
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Guo, Xiaolong
Yu, Hua
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Yu, Hua
Xiong, Junzhi
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Xiong, Junzhi
Dai, Qian
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Dai, Qian
Li, Yuanyuan
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Li, Yuanyuan
Zhang, Wei
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Dalian Med Univ, Affiliated Hosp 1, Dept Orthoped, Dalian, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Zhang, Wei
Liao, Xiping
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Liao, Xiping
He, Xiaomei
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
He, Xiaomei
Zhou, Hongli
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
Zhou, Hongli
Zhang, Kebin
论文数: 0引用数: 0
h-index: 0
机构:
Army Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R ChinaArmy Med Univ, Xinqiao Hosp, Clin Med Res Ctr, Chongqing, Peoples R China
机构:
Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USALawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA
Rajeev, Lara
Garber, Megan E.
论文数: 0引用数: 0
h-index: 0
机构:
Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA
Univ Calif Berkeley, Dept Comparat Biochem, Berkeley, CA 94720 USALawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA
Garber, Megan E.
Mukhopadhyay, Aindrila
论文数: 0引用数: 0
h-index: 0
机构:
Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA
Univ Calif Berkeley, Dept Comparat Biochem, Berkeley, CA 94720 USA
Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USALawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA