Pseudomonas aeruginosa two-component system CprRS regulates HigBA expression and bacterial cytotoxicity in response to LL-37 stress
被引:4
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作者:
Song, Yingjie
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机构:
Sichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Song, Yingjie
[1
]
Zhang, Siping
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机构:
Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Zhang, Siping
[2
]
Zhao, Ninglin
论文数: 0引用数: 0
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机构:
Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Zhao, Ninglin
[3
]
Nong, Cheng
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机构:
Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Nong, Cheng
[3
]
He, Yongxing
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机构:
Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
He, Yongxing
[2
]
Bao, Rui
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Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
Bao, Rui
[3
]
机构:
[1] Sichuan Normal Univ, Coll Life Sci, Chengdu, Peoples R China
[2] Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou, Peoples R China
[3] Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu, Peoples R China
Pseudomonas aeruginosa is a highly pathogenic bacterium known for its ability to sense and coordinate the production of virulence factors in response to host immune responses. However, the regulatory mechanisms underlying this process have remained largely elusive. In this study, we investigate the two-component system CprRS in P. aeruginosa and unveil the crucial role of the sensor protein CprS in sensing the human host defense peptide LL-37, thereby modulating bacterial virulence. We demonstrate that CprS acts as a phosphatase in the presence of LL-37, leading to the phosphorylation and activation of the response regulator CprR. The results prove that CprR directly recognizes a specific sequence within the promoter region of the HigBA toxin-antitoxin system, resulting in enhanced expression of the toxin HigB. Importantly, LL-37-induced HigB expression promotes the production of type III secretion system effectors, leading to reduced expression of proinflammatory cytokines and increased cytotoxicity towards macrophages. Moreover, mutations in cprS or cprR significantly impair bacterial survival in both macrophage and insect infection models. This study uncovers the regulatory mechanism of the CprRS system, enabling P. aeruginosa to detect and respond to human innate immune responses while maintaining a balanced virulence gene expression profile. Additionally, this study provides new evidence and insights into the complex regulatory system of T3SS in P. aeruginosa within the host environment, contributing to a better understanding of host-microbe communication and the development of novel strategies to combat bacterial infections.
机构:
Univ Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USA
E Tennessee State Univ, Johnson City, TN 37614 USAUniv Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USA
Pritchett, Christopher L.
Little, Alexander S.
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Univ Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USAUniv Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USA
Little, Alexander S.
Okkotsu, Yuta
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Univ Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USAUniv Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USA
Okkotsu, Yuta
Frisk, Anders
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Tulane Univ, Hlth Sci Ctr, New Orleans, LA 70118 USAUniv Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USA
Frisk, Anders
Cody, William L.
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Univ Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USAUniv Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USA
Cody, William L.
Covey, Christopher R.
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Univ Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USAUniv Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USA
Covey, Christopher R.
Schurr, Michael J.
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Univ Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USAUniv Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO 80045 USA
机构:
Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
Zhao, Ning-Lin
Zhu, Zi-Qi
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Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
Zhu, Zi-Qi
Feng, Han-Zhong
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机构:
Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
Feng, Han-Zhong
Song, Ying-Jie
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机构:
Sichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
Song, Ying-Jie
Huang, Qin
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Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
Huang, Qin
Mou, Xing-Yu
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Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
Mou, Xing-Yu
Nong, Cheng
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Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
Nong, Cheng
He, Yong-Xing
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机构:
Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
He, Yong-Xing
Bao, Rui
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Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
机构:
Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Xiao, Qian
Luo, Yanfen
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Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Luo, Yanfen
Shi, Wen
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Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Shi, Wen
Lu, Yang
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Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Lu, Yang
Xiong, Rui
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机构:
Sun Yat Sen Univ, Dept Lab Med, Affiliated Hosp 1, Guangzhou, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Xiong, Rui
Wu, Xinggui
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Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Wu, Xinggui
Huang, Haihao
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Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Huang, Haihao
Zhao, Chanjing
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机构:
Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Zhao, Chanjing
Zeng, Jianming
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h-index: 0
机构:
Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Zeng, Jianming
Chen, Cha
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Guangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
Guangzhou Univ Chinese Med, Clin Coll 2, 55 Inner Ring West Rd, Guangzhou 510006, Peoples R ChinaGuangdong Hosp Tradit Chinese Med, Dept Lab Med, Guangzhou 510120, Peoples R China
机构:
Sichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R China
Song, Yingjie
Wu, Xiyu
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Sichuan Univ, West China Hosp, Adv Mass Spectrometry Ctr, Res Core Facil,Frontiers Sci Ctr Dis Related Mol N, Chengdu 610213, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R China
Wu, Xiyu
Li, Ze
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Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R China
Li, Ze
Ma, Qin qin
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Sichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R China
Ma, Qin qin
Bao, Rui
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Sichuan Univ, West China Hosp, Ctr Infect Dis, Div Infect Dis,State Key Lab Biotherapy, Chengdu 610041, Peoples R ChinaSichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R China