The purpose of this study is to explore how corn varieties and moisture content affect the mechanical properties of corn with high moisture content (26.0 similar to 36.4%). The mechanical properties of corn that affect corn threshing quality are determined by establishing the contact mechanical model of the threshing elements and corn kernels. These mechanical properties of corn include the size characteristics of corn (corn ear weight and corn kernel triaxial size) in addition to the mechanical characteristics of corn (corn elastic modulus, corn kernel breaking force, and corn stalk breaking force). The effects of corn varieties and moisture content on the mechanical properties of corn were tested. The results showed that the corn varieties and moisture content had significant effects on the mechanical properties of corn. Among them, the different levels of moisture content levels have significant differences in the mechanical properties of corn, while corn varieties have different significant differences in the mechanical properties of corn. The ear weight, corn kernel triaxial size, and the axial and radial breaking force of corn pedicels all increased significantly as the moisture content increased. Corn's elastic modulus, kernel breaking force, and the normal breaking force of corn pedicels all decreased significantly as moisture content increased. The breaking force of corn kernels at different positions is in the order of ventral, lateral, and top surface, and the breaking force of corn pedicels in different directions is in the order of normal, axial, and radial. When the moisture content is between 26.0% and 36.4%, the maximum breaking force of a corn kernel is 401.62 N, and the minimum is 35.47 N. The maximum breaking force of corn pedicels is 32.72 N, and the minimum is 5.37 N. Understanding the influences of these relationships is very important for applications related to the threshing process, such as selecting the threshing mode, designing the threshing mechanism, and setting the threshing parameters.