The COMPASS Complex Regulates Fungal Development and Virulence through Histone Crosstalk in the Fungal Pathogen Cryptococcus neoformans

被引：5
|
作者：
Liu, Ruoyan [1 ]
Chen, Xiaoyu [1 ]
Zhao, Fujie [1 ]
Jiang, Yixuan [1 ]
Lu, Zhenguo [1 ]
Ji, Huining [2 ]
Feng, Yuanyuan [2 ]
Li, Junqiang [1 ]
Zhang, Heng [1 ]
Zheng, Jianting [2 ,3 ]
Zhang, Jing [1 ]
Zhao, Youbao [1 ]
机构：
[1] Henan Agr Univ, Coll Vet Med, Zhengzhou 450046, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
[3] Shanghai Jiao Tong Univ, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China
来源：
JOURNAL OF FUNGI | 2023年 / 9卷 / 06期
基金：
中国国家自然科学基金;
关键词：
COMPASS; H2B ubiquitination; Cryptococcus neoformans; yeast-to-hypha transition; virulence; H3K4; METHYLATION; HYPHAL GROWTH; C; NEOFORMANS; TITAN CELL; METHYLTRANSFERASE; TERMINATION; HOMOLOG; FAMILY; DOMAIN; LEVEL;
D O I：
10.3390/jof9060672
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The Complex of Proteins Associated with Set1 (COMPASS) methylates lysine K4 on histone H3 (H3K4) and is conserved from yeast to humans. Its subunits and regulatory roles in the meningitis-causing fungal pathogen Cryptococcus neoformans remain unknown. Here we identified the core subunits of the COMPASS complex in C. neoformans and C. deneoformans and confirmed their conserved roles in H3K4 methylation. Through AlphaFold modeling, we found that Set1, Bre2, Swd1, and Swd3 form the catalytic core of the COMPASS complex and regulate the cryptococcal yeast-to-hypha transition, thermal tolerance, and virulence. The COMPASS complex-mediated histone H3K4 methylation requires H2B mono-ubiquitination by Rad6/Bre1 and the Paf1 complex in order to activate the expression of genes specific for the yeast-to-hypha transition in C. deneoformans. Taken together, our findings demonstrate that putative COMPASS subunits function as a unified complex, contributing to cryptococcal development and virulence.
引用
收藏
页数：21
相关论文
共 50 条
  • [21] Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis
    Nelson, RT
    Hua, J
    Pryor, B
    Lodge, JK
    GENETICS, 2001, 157 (03) : 935 - 947
  • [22] Discovery of CO2 tolerance genes associated with virulence in the fungal pathogen Cryptococcus neoformans
    Chadwick, Benjamin J.
    Ristow, Laura C.
    Xie, Xiaofeng
    Krysan, Damian J.
    Lin, Xiaorong
    NATURE MICROBIOLOGY, 2024, 9 (10): : 2684 - 2695
  • [23] Cyclic AMP-dependent protein kinase controls virulence of the fungal pathogen Cryptococcus neoformans
    D'Souza, CA
    Alspaugh, JA
    Yue, CL
    Harashima, T
    Cox, GM
    Perfect, JR
    Heitman, J
    MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (09) : 3179 - 3191
  • [24] Erg6 affects membrane composition and virulence of the human fungal pathogen Cryptococcus neoformans
    Oliveira, Fabiana Freire M.
    Paes, Hugo Costa
    Peconick, Luisa Defranco F.
    Fonseca, Fernanda L.
    Freitas Marina, Clara Luna
    Bocca, Anamelia Lorenzetti
    Homem-de-Mello, Mauricio
    Rodrigues, Marcio Lourenco
    Albuquerque, Patricia
    Nicola, Andre Moraes
    Alspaugh, J. Andrew
    Felipe, Maria Sueli S.
    Fernandes, Larissa
    FUNGAL GENETICS AND BIOLOGY, 2020, 140
  • [25] Thiol peroxidase is critical for virulence and resistance to nitric oxide and peroxide in the fungal pathogen, Cryptococcus neoformans
    Missall, TA
    Pusateri, ME
    Lodge, JK
    MOLECULAR MICROBIOLOGY, 2004, 51 (05) : 1447 - 1458
  • [26] The fungal pathogen Cryptococcus neoformans manipulates macrophage phagosome maturation
    Smith, Leanne M.
    Dixon, Emily F.
    May, Robin C.
    CELLULAR MICROBIOLOGY, 2015, 17 (05) : 702 - 713
  • [27] Cryptococcus neoformans Copper Detoxification Machinery Is Critical for Fungal Virulence
    Ding, Chen
    Festa, Richard A.
    Chen, Ying-Lien
    Espart, Anna
    Palacios, Oscar
    Espin, Jordi
    Capdevila, Merce
    Atrian, Silvia
    Heitman, Joseph
    Thiele, Dennis J.
    CELL HOST & MICROBE, 2013, 13 (03) : 265 - 276
  • [28] A nuclear-encoded intein in the fungal pathogen Cryptococcus neoformans
    Butler, MI
    Goodwin, TJD
    Poulter, RTM
    YEAST, 2001, 18 (15) : 1365 - 1370
  • [29] Characterization of the MFα pheromone of the human fungal pathogen Cryptococcus neoformans
    Davidson, RC
    Moore, TDE
    Odom, AR
    Heitman, J
    MOLECULAR MICROBIOLOGY, 2000, 38 (05) : 1017 - 1026
  • [30] Thioredoxin reductase is essential for viability in the fungal pathogen Cryptococcus neoformans
    Missall, TA
    Lodge, JK
    EUKARYOTIC CELL, 2005, 4 (02) : 487 - 489
12345