Nutritional requirements of human induced pluripotent stem cells

被引：7

作者：

Lyra-Leite, Davi M. ^{[1
,2
]}

Copley, Raymond R. ^{[3
]}

Freeman, Phillip P. ^{[3
]}

Pongpamorn, Praeploy ^{[1
,2
]}

Shah, Disheet ^{[1
,2
]}

McKenna, Donald E. ^{[3
]}

Lenny, Brian ^{[4
]}

Pinheiro, Emily A. ^{[1
,2
]}

Weddle, Carly J. ^{[1
,2
]}

Gharib, Mennat ^{[1
,2
]}

Javed, Hoor ^{[1
,2
]}

Fonoudi, Hananeh ^{[1
,2
]}

Sapkota, Yadav ^{[4
]}

Burridge, Paul W. ^{[1
,2
]}

机构：

[1] Northwestern Univ, Dept Pharmacol, Feinberg Sch Med, Chicago, IL 60611 USA

[2] Northwestern Univ, Ctr Pharmacogen, Feinberg Sch Med, Chicago, IL 60611 USA

[3] Clever Carnivore Inc, Chicago, IL 60614 USA

[4] St Jude Childrens Res Hosp, Dept Epidemiol & Canc Control, Memphis, TN 38105 USA

来源：

STEM CELL REPORTS | 2023年 / 18卷 / 06期

关键词：

MOLECULAR GROWTH REQUIREMENTS; DIPLOID CHINESE-HAMSTER; MAMMALIAN CELLS; CLONAL GROWTH; TISSUE CULTURE; HELA CELLS; ACID; DERIVATION; GLUTAMINE; NEURONS;

D O I：

10.1016/j.stemcr.2023.05.004

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The nutritional requirements for human induced pluripotent stem cell (hiPSC) growth have not been extensively studied. Here, building on our prior work that established the suitable non-basal medium components for hiPSC growth, we develop a simplified basal medium consisting of just 39 components, demonstrating that many ingredients of DMEM/F12 are either not essential or are at suboptimal concentrations. This new basal medium along with the supplement, which we call BMEM, enhances the growth rate of hiPSCs over DMEM/ F12-based media, supports derivation of multiple hiPSC lines, and allows differentiation to multiple lineages. hiPSCs cultured in BMEM consistently have enhanced expression of undifferentiated cell markers such as POU5F1 and NANOG, along with increased expression of markers of the primed state and reduced expression of markers of the naive state. This work describes titration of the nutritional requirements of human pluripotent cell culture and identifies that suitable nutrition enhances the pluripotent state.

引用

页码：1371 / 1387

页数：17

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