Application of CRISPR-Cas9 gene editing technology in basic research, diagnosis and treatment of colon cancer

被引:9
|
作者
Meng, Hui [1 ]
Nan, Manman [1 ]
Li, Yizhen [1 ]
Ding, Yi [1 ]
Yin, Yuhui [1 ]
Zhang, Mingzhi [2 ]
机构
[1] Zhengzhou Univ, Dept Pathol, Affiliated Hosp 1, Zhengzhou, Henan, Peoples R China
[2] Zhengzhou Univ, Dept Oncol, Affiliated Hosp 1, Zhengzhou, Henan, Peoples R China
来源
关键词
CRISPR tools; genome editing; colorectal carcinoma; Genetic tool; basic research; EPITHELIAL-MESENCHYMAL TRANSITION; COLORECTAL-CANCER; CELL-SURVIVAL; GENOMIC DNA; IN-VITRO; BINDING; PROLIFERATION; TARGET; RNA; OVEREXPRESSION;
D O I
10.3389/fendo.2023.1148412
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Colon cancer is the fourth leading cause of cancer death worldwide, and its progression is accompanied by a complex array of genetic variations. CRISPR/Cas9 can identify new drug-resistant or sensitive mutations in colon cancer, and can use gene editing technology to develop new therapeutic targets and provide personalized treatments, thereby significantly improving the treatment of colon cancer patients. CRISPR/Cas9 systems are driving advances in biotechnology. RNA-directed Cas enzymes have accelerated the pace of basic research and led to clinical breakthroughs. This article reviews the rapid development of CRISPR/Cas in colon cancer, from gene editing to transcription regulation, gene knockout, genome-wide CRISPR tools, therapeutic targets, stem cell genomics, immunotherapy, metabolism-related genes and inflammatory bowel disease. In addition, the limitations and future development of CRISPR/Cas9 in colon cancer studies are reviewed. In conclusion, this article reviews the application of CRISPR-Cas9 gene editing technology in basic research, diagnosis and treatment of colon cancer.
引用
收藏
页数:21
相关论文
共 50 条
  • [21] Gene Editing of Human Hepatocytes by CRISPR-Cas9
    Zhang, Qingshuo
    Tiyaboonchai, Amita
    Balaji, Niveditha
    Naugler, Willscott
    Grompe, Markus
    MOLECULAR THERAPY, 2018, 26 (05) : 367 - 367
  • [22] CRISPR-cas9 Gene Editing for Cystic Fibrosis
    Xia, Emily
    MOLECULAR THERAPY, 2019, 27 (04) : 196 - 196
  • [23] CRISPR-Cas9 gene editing and human diseases
    Jinka, Chaitra
    Sainath, Chithirala
    Babu, Shyamaladevi
    Chakravarthi, Chennupati Ashok
    Prasanna, Muppidi Lakshmi
    Krishnan, Madhan
    Sekar, Gayathri
    Chinnaiyan, Mayilvanan
    Kumari, Andugula Swapna
    BIOINFORMATION, 2022, 18 (11) : 1081 - 1086
  • [24] Genome Editing of a Secondary Alcohol Dehydrogenase Gene by CRISPR-Cas9 Technology
    Huang, Jenq-Kuen
    Samassekou, Kadidia
    McClenahan, Shawn
    Seiver, Jacob
    Holt, Scott
    Wen, Lisa
    FASEB JOURNAL, 2019, 33
  • [25] CRISPR-Cas9 gene editing for patients with haemoglobinopathies
    不详
    LANCET HAEMATOLOGY, 2019, 6 (09): : E438 - E438
  • [26] Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology
    Qiu, Yan
    Ding, Qiurong
    STAR PROTOCOLS, 2021, 2 (01):
  • [27] Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli
    Zhang, Li
    Meng, Sufang
    Liu, Yumei
    Han, Fengqing
    Xu, Tiemin
    Zhao, Zhiwei
    Li, Zhansheng
    GENES, 2024, 15 (06)
  • [28] Development and application of CRISPR-Cas9 for genome editing
    Zhang, Feng
    TRANSGENIC RESEARCH, 2014, 23 (05) : 842 - 842
  • [29] A REVIEW ON GENOME EDITING BY CRISPR-CAS9 TECHNIQUE FOR CANCER TREATMENT
    Khan, M. Niuz Morshed
    Islam, K. Khaldun
    Ashraf, A.
    Barman, N. Chandra
    WORLD CANCER RESEARCH JOURNAL, 2020, 7
  • [30] Application of the gene editing tool, CRISPR-Cas9, for treating neurodegenerative diseases
    Kolli, Nivya
    Lu, Ming
    Maiti, Panchanan
    Rossignol, Julien
    Dunbar, Gary L.
    NEUROCHEMISTRY INTERNATIONAL, 2018, 112 : 187 - 196