Immobilized Cellulase on NH2-MIL-88(Fe) and Its Performance as a Biocatalyst

被引：0
|
作者：
Jiang, Jing [1 ]
Gong, Xiaowu [1 ]
Li, Tiantian [1 ]
Huang, Jin [1 ,2 ,3 ,4 ]
Zhou, Na [1 ]
Jia, Xin [1 ]
机构：
[1] Shihezi Univ, Sch Chem & Chem Engn, State Key Lab Incubat Base Green Proc Chem Engn, Shihezi 832003, Peoples R China
[2] Southwest Univ, Sch Chem & Chem Engn, Chongqing, Peoples R China
[3] Southwest Univ, Chongqing Key Lab Soft Matter Mat Chem & Funct Mfg, Chongqing, Peoples R China
[4] Southwest Univ, Belt & Rd B&R Internat Joint Res Lab Sustainable M, Chongqing, Peoples R China
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2024年 / 196卷 / 08期
基金：
中国国家自然科学基金;
关键词：
Immobilized cellulase; NH2-MIL-88(Fe); Enzyme reusability; Catalytic performance; Corn cob; FE3O4; NANOPARTICLES; HYDROLYSIS; CHAPERONES; STRATEGIES;
D O I：
10.1007/s12010-023-04759-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To broaden pH range and improve thermal stability, reusability, storage stability, and organic solvent tolerance of natural enzymes, a magnetic material (NH2-MIL-88(Fe)) was synthesized as a new material to immobilize cellulase. The results showed that the optimal temperature and pH of cellulase immobilized on NH2-MIL-88(Fe) showed a wider range compared to free cellulase, and 74% and 83% of the initial activity could be retained after 10 cycles and storage for 49 days, respectively. Moreover, the tolerance for organic solvents was improved compared with free enzyme. The reducing sugar yields from sodium carboxymethylcellulose (CMC) and corn cob hydrolyzed with cellulase immobilized on NH2-MIL-88(Fe) were higher than observed with the free enzyme, which demonstrated the better biocatalytic performance of cellulase immobilized on NH2-MIL-88(Fe).
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页码：4745 / 4758
页数：14
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