Simultaneous quantification and structural characterization of monoclonal antibodies after administration using capillary zone electrophoresis-tandem mass spectrometry

被引:4
|
作者
Reinert, Tessa [1 ,2 ]
Gahoual, Rabah [2 ]
Mignet, Nathalie [2 ]
Kulus, Alexandre [1 ]
Allez, Matthieu [4 ]
Houze, Pascal [2 ,3 ]
Francois, Yannis-Nicolas [1 ]
机构
[1] Univ Strasbourg, Lab Spectrometrie Masse Interact & Syst LSMIS UMR, Strasbourg, France
[2] Univ Paris Cite, Unite Technol Chim & Biol Sante UTCBS, CNRS, Inserm,Fac Sci Pharmaceut & Biol, Paris, France
[3] Hop Lariboisiere, Assistance Publ Hop Paris AP HP, Lab Toxicol Biol, Paris, France
[4] Hop St Louis, Assistance Publ Hop Paris AP HP, Inserm, U1160, Paris, France
关键词
Capillary electrophoresis; Mass Spectrometry; Monoclonal antibodies; Quantification; Biological sample; Post-translational modifications; TUMOR-NECROSIS-FACTOR; CROHNS-DISEASE; METHIONINE OXIDATION; INFLIXIMAB TREATMENT; BIOLOGICAL-ACTIVITY; HUMAN IGG1; DEAMIDATION; IMPACT; GLYCOSYLATION; PROTEOMICS;
D O I
10.1016/j.jpba.2023.115446
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Monoclonal antibodies (mAbs) are demonstrating major success in various therapeutic areas such as oncology and the treatment of immune disorders. Over the past two decades, novel analytical methodologies allowed to address the challenges of mAbs characterization in the context of their production. However, after administration only their quantification is performed and insights regarding their structural evolution remain limited. For instance, clinical practice has recently highlighted significant inter-patient differences in mAb clearance and unexpected clinical responses, without providing alternative interpretations. Here, we report the development of a novel analytical strategy based on capillary zone electrophoresis coupled to tandem mass spectrometry (CEMS/MS) for the simultaneous absolute quantification and structural characterization of infliximab (IFX) in human serum. CE-MS/MS quantification was validated over the range 0.4-25 mu g center dot mL(-1) corresponding to the IFX therapeutic window and achieved a LOQ of 0.22 mu g center dot mL(-1) (1.5 nM) while demonstrating outstanding specificity compared to the ELISA assay. CE-MS/MS allowed structural characterization and estimation of the relative abundance of the six major N-glycosylations expressed by IFX. In addition, the results allowed characterization and determination of the level of modification of post-translational modifications (PTMs) hotspots including deamidation of 4 asparagine and isomerization of 2 aspartate. Concerning N-glycosylation and PTMs, a new normalization strategy was developed to measure the variation of modification levels that occur strictly during the residence time of IFX in the patient's system, overcoming artefactual modifications induced by sample treatment and/or storage. The CE-MS/MS methodology was applied to the analysis of samples from patients with Crohn's disease. The data identified a gradual deamidation of a particular asparagine residue located in the complementary determining region that correlated with IFX residence time, while the evolution of IFX concentration showed significant variability among patients.
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页数:11
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