DNA methylation and gene expression in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate

被引：0
|
作者：
Wang, Quankai [1 ,3 ]
Wang, Boshen [1 ,2 ]
Li, Huanhuan [1 ,3 ]
Wang, Miao [1 ]
Li, Xinwei [1 ]
Zhu, Baoli [2 ]
Xu, Jianning [1 ,3 ,4 ]
机构：
[1] Natl Inst Occupat Hlth & Poison Control, Chinese Ctr Dis Control & Prevent, Beijing, Peoples R China
[2] Jiangsu Ctr Dis Control & Prevent, Nanjing, Peoples R China
[3] Chinese Ctr Dis Control & Prevent, Key Lab Chem Safety & Hlth, Beijing, Peoples R China
[4] Natl Inst Occupat Hlth & Poison Control, Chinese Ctr Dis Control & Prevent, Beijing 100050, Peoples R China
来源：
ALL LIFE | 2023年 / 16卷 / 01期
基金：
中国国家自然科学基金;
关键词：
DNA methylation; glycidyl methacrylate; 16HBE; CANCER; TRPV1;
D O I：
10.1080/26895293.2023.2206543
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to elucidate the role of DNA methylation-silenced genes in malignant transformation. We screened DNA methylation-silenced genes during methacrylate (GMA)-induced malignant transformation of human bronchial epithelial cells (16HBE) by DNA methylation microarray analysis. The methylation status of some identified genes was assessed by a polymerase chain reaction and compared with uninduced control cells. A total of 801, 66 and 30 genes were silenced in 10th, 20th and 30th generation cells; 8 genes were silenced in 10th and 20th generation cells but not in 30th generation cells; 14 genes were silenced in 30th and 10th generation cells but not in 20th generation cells; and one gene was silenced in 20th and 30th generation cells but not in 10th generation cells. TRPV1 and SOD2 genes were methylated and their gene expression levels were significantly lower in 30th generation 16HBE cells treated with GMA compared to uninduced control cells (P < 0.05). The expression levels of DNA methylation-silenced genes were altered throughout the GMA-induced malignant transformation of 16HBE cells. Furthermore, TRPV1/SOD2 may represent a specific molecular marker associated with the malignant transformation of GMA-stimulated 16HBE cells.
引用
收藏
页数：10
相关论文
共 50 条
  • [41] Mechanisms of radiation-induced neoplastic transformation of human bronchial epithelial cells
    Zhao, YL
    Piao, CQ
    Hall, EJ
    Hei, TK
    RADIATION RESEARCH, 2001, 155 (01) : 230 - 234
  • [42] Controls of EGF-induced morphological transformation of human bronchial epithelial cells
    Beckmann, JD
    Stewart, A
    Kai, M
    Keeton, TP
    JOURNAL OF CELLULAR PHYSIOLOGY, 2001, 189 (02) : 171 - 178
  • [43] Cadmium-induced malignant transformation of human prostate epithelial cells
    Achanzar, WE
    Diwan, BA
    Liu, J
    Quader, ST
    Webber, MM
    Waalkes, MP
    CANCER RESEARCH, 2001, 61 (02) : 455 - 458
  • [44] Expression of cytokines on human bronchial epithelial cells induced by influenza virus A
    Adachi, M
    Matsukura, S
    Tokunaga, H
    Kokubu, F
    INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1997, 113 (1-3) : 307 - 311
  • [45] THIOPURINE INDUCED DISTURBANCE OF DNA METHYLATION IN HUMAN-MALIGNANT CELLS
    DEABREU, R
    LAMBOOY, L
    STET, E
    VOGELSMENTINK, T
    VANDENHEUVEL, L
    ADVANCES IN ENZYME REGULATION, VOL 35, 1995, 35 : 251 - 263
  • [46] Identification of miRNAs and Its Target Gene Network in Mediating Arsenite-induced Malignant Transformation in Human Bronchial Epithelial Cells by miRNA Microarray Analysis
    Zhang, Z.
    Gu, S.
    Dai, H.
    Sun, D.
    Lai, Y.
    Liu, Y.
    ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2017, 58 : S70 - S71
  • [47] Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression
    Gordon, Katrina
    Clouaire, Thomas
    Bao, Xun X.
    Kemp, Sadie E.
    Xenophontos, Maria
    de Las Heras, Jose Ignacio
    Stancheva, Irina
    NUCLEIC ACIDS RESEARCH, 2014, 42 (06) : 3529 - 3541
  • [48] Regulation of collagen gene expression in bronchial myofibroblasts by human primary bronchial epithelial cells.
    Zhang, S
    Patel, HP
    Holgate, ST
    Roche, WR
    JOURNAL OF PATHOLOGY, 2000, 190 : 54A - 54A
  • [49] Molecular Mechanisms of Malignant Transformation by Low Dose Cadmium in Normal Human Bronchial Epithelial Cells
    Cartularo, Laura
    Kluz, Thomas
    Cohen, Lisa
    Shen, Steven S.
    Costa, Max
    PLOS ONE, 2016, 11 (05):
  • [50] Effects of PM2.5 on the Transcriptome and DNA Methylation Machinery of Human Bronchial Epithelial Cells
    Tripathi, P.
    Koneva, L.
    Deng, F.
    Chen, Y.
    Huang, S. K.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2018, 197
12345