Complementary charge-driven encapsulation of functional protein by engineered protein cages in cellulo

被引：1

作者：

Zakaszewski, Daniel ^{[1
,2
]}

Koziej, Lukasz ^{[1
]}

Pankowski, Jedrzej ^{[1
,3
]}

Malolan, V. Vishal ^{[1
,2
]}

Gamperli, Nina ^{[4
]}

Heddle, Jonathan G. G. ^{[1
]}

Hilvert, Donald ^{[4
]}

Azuma, Yusuke ^{[1
,4
]}

机构：

[1] Jagiellonian Univ, Malopolska Ctr Biotechnol MCB, Gronostajowa 7A, PL-30387 Krakow, Poland

[2] Jagiellonian Univ, Doctoral Sch Exact & Nat Sci, Prof S Lojasiewicza 11, PL-30348 Krakow, Poland

[3] Jagiellonian Univ, Fac Biochem, Biophys, Sand Biotechnol, Gronostajowa 7, PL-30387 Krakow, Poland

[4] Swiss Fed Inst Technol, Lab Organ Chem, CH-8093 Zurich, Switzerland

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2023年 / 11卷 / 28期

关键词：

EVOLUTION; CARGO; FERRITIN; MICROCOMPARTMENT; MECHANISM; PROTEASES; ENZYMES;

D O I：

10.1039/d3tb00754e

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Charge-driven inclusion complex formation in live cells was examined using a degradation-prone fluorescent protein and a series of protein cages. The results show that sufficiently strong host-guest ionic interaction and an intact shell-like structure are crucial for the protective guest encapsulation.

引用

页码：6540 / 6546

页数：7

共 50 条

[41] Protein-Engineered Hydrogel Encapsulation for 3-D Culture of Murine Cochlea
Chang, David T.
Chai, Renjie
DiMarco, Rebecca
Heilshorn, Sarah C.
Cheng, Alan G.
OTOLOGY & NEUROTOLOGY, 2015, 36 (03) : 531 - 538
[42] Role of Surface Charge Density in Nanoparticle-Templated Assembly of Bromovirus Protein Cages
Daniel, Marie-Christine
Tsvetkova, Irina B.
Quinkert, Zachary T.
Murali, Ayaluru
De, Mrinmoy
Rotello, Vincent M.
Kao, C. Cheng
Dragnea, Bogdan
ACS NANO, 2010, 4 (07) : 3853 - 3860
[43] High-capacity functional protein encapsulation in nanoengineered polypeptide microcapsules
Zhi, ZL
Haynie, DT
CHEMICAL COMMUNICATIONS, 2006, (02) : 147 - 149
[44] A Model for the Molecular Mechanism of an Engineered Light-Driven Protein Machine
Hoersch, Daniel
Kortemme, Tanja
STRUCTURE, 2016, 24 (04) : 576 - 584
[45] BIOT 174-Effects of protein charge anisotropy in electrostatically-driven protein aggregation
Dubin, Paul L.
Xu, Yisheng
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2009, 238
[46] Highly Sensitive Naphthalene-Based Twisted Intramolecular Charge Transfer Molecules for the Detection of In Vitro and In Cellulo Protein Aggregates
Debnath, Joy
Sekar, Yuvasree
Bera, Anwesha
ACS MEDICINAL CHEMISTRY LETTERS, 2024, 15 (12): : 2129 - 2132
[47] Protein-RNA complexation driven by the charge regulation mechanism
Barroso da Silva, Fernando Luis
Derreumaux, Philippe
Pasquali, Samuela
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 498 (02) : 264 - 273
[48] Dominant effect of protein charge rather than protein shape in apparent DNA bending by engineered bZIP domains
Hardwidge, PR
Kahn, JD
Maher, LJ
BIOCHEMISTRY, 2002, 41 (26) : 8277 - 8288
[49] Al-Driven Protein Discovery for Functional Ingredients
Varadan, Ranjani
CHEMICAL ENGINEERING PROGRESS, 2023, 119 (09) : 40 - 41
[50] Ai-driven protein discovery for Functional ingredients
Varadan, Ranjani
Chemical Engineering Progress, 2023, (September) : 40 - 43

← 1 2 3 4 5 →