Possible mitigating effect of adropin on lung injury in diabetic rats: Targeting the role of Rho A/Rho-associated kinase pathway

被引：4
|
作者：
Rizk, Fatma H. [1 ]
El-Saka, Mervat H. [1 ]
Ibrahim, Rowida Raafat [2 ,7 ]
El-Deeb, Omnia Safwat [2 ]
Ibrahim, Hoda A. [2 ]
El Saadany, Amira A. [3 ]
Mashal, Shaimaa S. [4 ]
Ammar, Leila [5 ]
Abdelsattar, Amal M. [6 ]
Barhoma, Ramez A. [1 ]
机构：
[1] Tanta Univ, Fac Med, Dept Physiol, Tanta, Egypt
[2] Tanta Univ, Fac Med, Dept Med Biochem & Mol Biol, Tanta, Egypt
[3] Tanta Univ, Fac Med, Dept Pharmacol, Tanta, Egypt
[4] Tanta Univ, Fac Med, Dept Internal Med, Tanta, Egypt
[5] Tanta Univ, Fac Med, Dept Histol, Tanta, Egypt
[6] Tanta Univ, Fac Med, Dept Anat, Tanta, Egypt
[7] Tanta Univ, Fac Med, Dept Med Biochem & Mol Biol, El Geesh St, Tanta, Egypt
来源：
BIOFACTORS | 2023年
关键词：
adropin; diabetic; hyperglycemia; lung injury; Rho A/ROCK pathway; MODEL; HYPERGLYCEMIA; GLUCOSE; CELLS; HOMEOSTASIS; METABOLISM; MECHANISMS; EXPRESSION; DISORDER; MELLITUS;
D O I：
10.1002/biof.1955
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This study evaluated possible mitigating effect of adropin on lung injury in diabetic rats, targeting role of Rho A/Rho-associated kinase pathway. Rats were allocated into four groups: control, adropin, diabetic, and diabetic+adropin groups. At the termination of the experiment, serum fasting glucose, insulin and adropin levels and insulin resistance were calculated. Wet/dry ratio, histopathological, immunohistochemical analyses, and relative real time gene expression of lung tissue was determined. Interleukin-6, tumor necrosis factor alpha, malondialdehyde, 8-Oxo-2'-deoxyguanosine, reduced glutathione, superoxide dismutase, Bcl-2, BAX, myeloperoxidase, intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and transforming growth factor-beta were determined in lung tissue. Adropin treatment in diabetic rats notably attenuated hyperglycemia and insulin resistance. Also, it mitigated diabetic lung injury via suppressing effect on Rho A/ROCK pathway, apoptosis, inflammatory reactions, oxidative stress, and fibrosis of lung tissue. Adropin can be considered as a promising therapeutic agent for treating diabetic lung injury.
引用
收藏
页码：928 / 939
页数：12
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