Identification and Profiling Analysis of microRNAs in Guava Fruit (Psidium guajava']java L.) and Their Role during Ripening

被引:0
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作者
Mejia-Mendoza, Mario Alejandro [1 ]
Garciduenas-Pina, Cristina [1 ]
Barrera-Figueroa, Blanca Estela [2 ]
Morales-Dominguez, Jose Francisco [1 ]
机构
[1] Univ Autonoma Aguascalientes UAA, Ctr Ciencias Basicas, Dept Quim, Ave Univ 940, Aguascalientes 20100, Mexico
[2] Univ Papaloapan, Ctr Invest Cient, Inst Biotecnol, Lab Biotecnol Vegetal, Circuito Cent 200,Parque Ind, Tuxtepec 68301, Mexico
关键词
climacteric fruit; ripening; gene regulation; transcription factors; microRNAs; guava; FAMILY-MEMBERS; EXPRESSION; GRAPEVINE; GENES;
D O I
10.3390/genes14112029
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The guava (Psidium guajava L.) is a climacteric fruit with an accelerated post-harvest overripening. miRNAs are small RNA sequences that function as gene regulators in eukaryotes and are essential for their survival and development. In this study, miRNA libraries were constructed, sequenced and analyzed from the breaker and ripe stages of guava fruit cv. Siglo XXI. One hundred and seventy-four mature miRNA sequences from 28 miRNA families were identified. The taxonomic distribution of the guava miRNAs showed a high level of conservation among the dicotyledonous plants. Most of the predicted miRNA target genes were transcription factors and genes involved in the metabolism of phytohormones such as abscisic acid, auxins, and ethylene, as revealed through an ontology enrichment analysis. The miRNA families miR168, miR169, miR396, miR397, and miR482 were classified as being directly associated with maturation, whereas the miRNA families miR160, miR165, miR167, miR3930, miR395, miR398, and miR535 were classified as being indirectly associated. With this study, we intended to increase our knowledge and understanding of the regulatory process involved in the ripening process, thereby providing valuable information for future research on the ripening of guava fruit.
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页数:15
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