Molecular detection of Brucella abortus and Brucella melitensis in domestic ruminants and their ticks in selected areas of western Iran

被引:2
|
作者
Moravedji, M. [1 ]
Beig, M. [2 ,3 ]
Baseri, N. [3 ,4 ]
Rahravani, M. [5 ]
Latifian, M. [6 ]
Esmaeili, S. [7 ,8 ]
机构
[1] Islamic Azad Univ, Fac Vet Med, Dept Clin Sci, Sanandaj Branch, Sanandaj, Iran
[2] Pasteur Inst Iran, Dept Bacteriol, Med Bacteriol, Tehran, Iran
[3] Pasteur Inst Iran, Med Bacteriol, Natl Reference Lab Plague, Tularemia & Q Fever Res Ctr Emerging & Reemerging, Hamadan, Iran
[4] Pasteur Inst Iran, Res Ctr Emerging & Reemerging Infect Dis, Dept Epidemiol & Biostat, Med Bacteriol, Tehran, Iran
[5] Razi Univ, Fac Vet Med, Dept Clin Sci, Kermanshah, Iran
[6] Pasteur Inst Iran, Tularemia & Q Fever Res Ctr Emerging & Reemerging, Med Lab Sci, Natl Reference Lab Plague, Hamadan, Iran
[7] Pasteur Inst Iran, Res Ctr Emerging & Reemerging Infect Dis, Dept Epidemiol & Biostat, Tehran, Iran
[8] Pasteur Inst Iran, Tularemia & Q Fever Res Ctr Emerging & Reemerging, Natl Reference Lab Plague, Hamadan, Iran
关键词
B; abortus; melitensis; Dermacentor marginatus; Rhipicephalus turanicus; Ruminant; REAL-TIME PCR; IDENTIFICATION; INFECTION; HUMANS; CATTLE;
D O I
10.22099/ijvr.2023.47192.6806
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Background: Brucellosis is one of the most important zoonotic diseases caused by Gram-negative bacteria belonging to the genus Brucella. Detection of Brucella species in different countries is of utmost importance. Aims: This study aimed to detect Brucella abortus and Brucella melitensis in domestic ruminant blood samples and their ticks in western Iran. Methods: Sampling was conducted on ruminants from August to September 2020 in four different counties of Kurdistan Province, including Divandareh, Marivan, Baneh, and Sanandaj. Totally, 250 blood samples were collected from 250 small ruminants. There were no ticks on the skin of six (2.4%) ruminants, and 244 ticks were isolated from 244 animals. After genomic DNA extraction from all the collected samples, quantitative polymerase chain reaction (qPCR) was performed to detect IS711 gene. Results: Based on qPCR results, Brucella genus was detected in two blood samples (0.8%) from female sheep and four ticks (1.6%) from male sheep, including three Dermacentor marginatus (1.22%) and one Rhipicephalus turanicus (0.4%). Although B. melitensis was not detected in any tick or blood sample, one tick sample (D. marginatus) was positive for B. abortus. Conclusion: Considering the positivity of ticks for brucellosis in this study, there is a possibility of Brucella transmission from infected ticks to humans and animals through tick bites, nevertheless, in order to identify the Brucella transmission relationship between ticks and animals, serological tests should be used in future studies.
引用
收藏
页码:270 / 275
页数:7
相关论文
共 50 条
  • [41] Molecular and serological survey of Brucella spp. among rodents in western Iran
    Hemati, Majid
    Khalili, Mohammad
    Esmaeili, Saber
    Mahmoudi, Ahmad
    Cohan, Hossein Ahangari
    Mostafavi, Ehsan
    BMC VETERINARY RESEARCH, 2025, 21 (01)
  • [42] COMPARISON OF MOLECULAR AND SEROLOGICAL TESTS FOR DETECTION OF BRUCELLA ABORTUS IN ASYMPTOMATIC BOVINE BREEDING BULLS
    Naz, S.
    Azeem, M.
    Hafeez, M. A.
    Ashraf, K.
    Asif, K.
    Ali, A.
    Ashfaq, H. A.
    Shehzad, W.
    Imran, M. S.
    Tayyub, M.
    Fareed, Z.
    JOURNAL OF ANIMAL AND PLANT SCIENCES, 2020, 30 (04): : 845 - 852
  • [43] Investigation into the sero-molecular prevalence of Brucella melitensis in small ruminants in districts Mohmand and Charsadda Khyber Pakhtunkhwa Pakistan
    Ul Hassan, Sohaib
    Khan, Farhan Anwar
    Shuaib, Muhammad
    Shahid, Muhammad
    Shah, Said Sajjad Ali
    Siddiqui, Shahrood Ahmed
    Pokoo-Aikins, Anthony
    Swelum, Ayman A.
    PLOS ONE, 2025, 20 (02):
  • [44] Detection and Segregation of Brucella abortus and Brucella melitensis in Aborted Bovine, Ovine, Caprine, Buffaloes and Came lid Fetuses by Application of Conventional and Real-time Polymerase Chain Reaction
    Dehkordi, Farhad Safarpoor
    Saberian, Shahin
    Momtaz, Hassan
    THAI JOURNAL OF VETERINARY MEDICINE, 2012, 42 (01): : 13 - 20
  • [45] Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds
    Babar Ali
    Muhammad Naeem
    Shakir Ullah
    Hamza Manzoor
    Muhammad Asif
    Nasir Mahmood
    Aliza Kanwal
    Mohammed Bourhia
    Musaab Dauelbait
    Turki M. Dawoud
    Adil Khan
    Ahmad Ali
    Furhan Iqbal
    Scientific Reports, 15 (1)
  • [46] Molecular detection of Brucella spp. in clinical samples of seropositive ruminants in Bosnia and Herzegovina
    Maksimovic, Zinka
    Jamakovic, Amela
    Semren, Orjana
    Rifatbegovic, Maid
    COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2022, 86
  • [47] Molecular Typing of Brucella melitensis and B. abortus From Human Blood Samples Using PCR-RFLP Method
    Mirnejad, Reza
    Mohammadi, Mozafar
    Majdi, Ali
    Taghizoghi, Niloufar
    Piranfar, Vahhab
    JUNDISHAPUR JOURNAL OF MICROBIOLOGY, 2013, 6 (06)
  • [48] Real-time multiplex PCR assay for detection of Brucella spp., B-abortus, and B-melitensis
    Probert, WS
    Schrader, MN
    Khuong, NY
    Bystrom, SL
    Graves, MH
    JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (03) : 1290 - 1293
  • [49] Molecular detection of Brucella species in patients suspicious of Brucellosis from Zanjan, Iran
    Garshasbi, Maryam
    Ramazani, Ali
    Sorouri, Rahim
    Javani, Siamak
    Moradi, Soheila
    BRAZILIAN JOURNAL OF MICROBIOLOGY, 2014, 45 (02) : 533 - 538
  • [50] Detection of Various Rickettsial Species in Ticks Collected from Small Ruminants in Western Iran
    Moravedji, Meysam
    Latifian, Mina
    Rahravani, Maryam
    Mostafavi, Ehsan
    Seyfi, Hamid
    Mohammadi, Mehrdad
    Mozoun, Mohammad Mahdi
    Stenos, John
    Esmaeili, Saber
    VECTOR-BORNE AND ZOONOTIC DISEASES, 2024, 24 (11) : 730 - 737