Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein

被引：0
|
作者：
Liu, Angela Rose [1 ]
Basavarajappa, Shrikanth C. [1 ]
Sarkar, Nandini [1 ]
Bruchez, Anna [1 ]
Ramakrishnan, Parameswaran [1 ,2 ,3 ]
机构：
[1] Case Western Reserve Univ, Sch Med, Dept Pathol, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Case Comprehens Canc Ctr, Sch Med, Cleveland, OH 44106 USA
[3] Case Western Reserve Univ, Sch Med, Dept Biochem, Cleveland, OH 44106 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 01期
关键词：
Cell-based Assays; Health Sciences; Immunology; Molecular Biology; Protein Biochemistry; Protein expression and purification;
D O I：
10.1016/j.xpro.2023.102127
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we provide a protocol for the design, expression, purification, and func-tional studies of an engineered trimeric version of the receptor-binding domain (tRBD) of SARS-CoV-2 spike protein. We describe the use of tRBD to block SARS-CoV-2 spike pseudovirus and true virus binding to cellular angiotensin converting enzyme-2 (ACE2), thereby blocking viral infection. This protocol is applicable to generate a trimeric version of any protein of interest.For complete details on the use and execution of this protocol, please refer to Basavarajappa et al. (2022).1
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页数：21
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