Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein

被引：0

作者：

Liu, Angela Rose ^{[1
]}

Basavarajappa, Shrikanth C. ^{[1
]}

Sarkar, Nandini ^{[1
]}

Bruchez, Anna ^{[1
]}

Ramakrishnan, Parameswaran ^{[1
,2
,3
]}

机构：

[1] Case Western Reserve Univ, Sch Med, Dept Pathol, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Case Comprehens Canc Ctr, Sch Med, Cleveland, OH 44106 USA

[3] Case Western Reserve Univ, Sch Med, Dept Biochem, Cleveland, OH 44106 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Cell-based Assays; Health Sciences; Immunology; Molecular Biology; Protein Biochemistry; Protein expression and purification;

D O I：

10.1016/j.xpro.2023.102127

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we provide a protocol for the design, expression, purification, and func-tional studies of an engineered trimeric version of the receptor-binding domain (tRBD) of SARS-CoV-2 spike protein. We describe the use of tRBD to block SARS-CoV-2 spike pseudovirus and true virus binding to cellular angiotensin converting enzyme-2 (ACE2), thereby blocking viral infection. This protocol is applicable to generate a trimeric version of any protein of interest.For complete details on the use and execution of this protocol, please refer to Basavarajappa et al. (2022).1

引用

页数：21

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