Metagenomic analysis of viromes in honey bee colonies (Apis mellifera; Hymenoptera: Apidae) after mass disappearance in Korea

被引:6
|
作者
Kwon, Minhyeok [1 ,2 ]
Jung, Chuleui [1 ,2 ]
Kil, Eui-Joon [1 ,2 ]
机构
[1] Andong Natl Univ, Dept Plant Med, Andong, South Korea
[2] Andong Natl Univ, Agr Sci & Technol Res Inst, Andong, South Korea
基金
新加坡国家研究基金会;
关键词
colony collapse disorder; high-throughput sequencing; honey bee viruses; virome analysis; honey bee (Apis mellifera L; PHYLOGENETIC ANALYSIS; VIRUS;
D O I
10.3389/fcimb.2023.1124596
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
After the nationwide, massive winter losses of honey bees in Korea during the winter of 2021, samplings were conducted from live honey bees in colonies and dead honey bees nearby colonies in the same bee-farms in six regions in Korea. Each sample was subjected to virome analysis using high-throughput sequencing technology. The number of viral reads was the lowest in the live honey bee group sample with 370,503 reads and the highest in the dead honey bee group sample with 42,659,622 reads. Viral contigs were matched with the viral genomes of the black queen cell virus, deformed wing virus, Israeli acute paralysis virus, and sacbrood virus, all of which have been previously reported in Korea. However, Apis rhabdovirus 5, bee macula-like virus, Varroa orthomyxovirus-1, Hubei partiti-like virus 34, Lake Sinai virus 2, 3, and 4, and the Ditton virus, were also discovered in this study, which are the first records in Korea. Plant viral sequences resembling those of Arabidopsis latent virus 1, and a novel viral sequence was also discovered. In the present study 55 complete viral genome sequences were identified. This study is the first virome analysis of domestic honey bees and provides the latest information on the diversity of honey bee viruses in Korea.
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页数:10
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