Approaching complete genomes, transcriptomes and epi-omes with accurate long-read sequencing

被引：34

作者：

Kovaka, Sam ^{[1
]}

Ou, Shujun ^{[1
,2
]}

Jenike, Katharine M. ^{[1
,3
]}

Schatz, Michael C. ^{[1
,3
,4
]}

机构：

[1] Johns Hopkins Univ, Dept Comp Sci, Baltimore, MD 21218 USA

[2] Ohio State Univ, Dept Mol Genet, Columbus, OH USA

[3] Johns Hopkins Univ, Dept Genet Med, Baltimore, MD 21218 USA

[4] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

来源：

NATURE METHODS | 2023年 / 20卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1038/s41592-022-01716-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The year 2022 will be remembered as the turning point for accurate long-read sequencing, which now establishes the gold standard for speed and accuracy at competitive costs. We discuss the key bioinformatics techniques needed to power long reads across application areas and close with our vision for long-read sequencing over the coming years.

引用

页码：12 / 16

页数：5

共 50 条

[31] Accurate detection of HBV splice variant DNA by using long-read sequencing
Hulspas, Sanne
Knetsch, Cornelis
Weber, Michiel
Hout, Anne
van Doorn, Leen-Jan
JOURNAL OF HEPATOLOGY, 2022, 77 : S750 - S750
[32] Genomics in the long-read sequencing era
van Dijk, Erwin L.
Naquin, Delphine
Gorrichon, Kevin
Jaszczyszyn, Yan
Ouazahrou, Rania
Thermes, Claude
Hernandez, Celine
TRENDS IN GENETICS, 2023, 39 (09) : 649 - 671
[33] Long-read sequencing for brain tumors
Shelton, William J.
Zandpazandi, Sara
Nix, J. Stephen
Gokden, Murat
Bauer, Michael
Ryan, Katie Rose
Wardell, Christopher P.
Vaske, Olena Morozova
Rodriguez, Analiz
FRONTIERS IN ONCOLOGY, 2024, 14
[34] Long-read sequencing in human genetics
Kraft, Florian
Kurth, Ingo
MEDIZINISCHE GENETIK, 2019, 31 (02) : 198 - 204
[35] Method of the year: long-read sequencing
Marx, Vivien
NATURE METHODS, 2023, 20 (01) : 6 - 11
[36] Reimagining Long-Read DNA Sequencing
不详
CHEMICAL ENGINEERING PROGRESS, 2017, 113 (10) : 28 - 28
[37] Long-read sequencing in fungal identification
Hoang, Minh Thuy Vi
Irinyi, Laszlo
Meyer, Wieland
MICROBIOLOGY AUSTRALIA, 2022, 43 (01) : 14 - 18
[38] Transcriptomics in the era of long-read sequencing
Monzo, Carolina
Liu, Tianyuan
Conesa, Ana
NATURE REVIEWS GENETICS, 2025,
[39] Long-read sequencing goes clinical
Neveling, K.
Derks, R.
Kwint, M.
van de Vorst, M.
Gardeitchik, T.
Nelen, M.
EUROPEAN JOURNAL OF HUMAN GENETICS, 2019, 27 : 516 - 516
[40] Method of the year: long-read sequencing
Vivien Marx
Nature Methods, 2023, 20 : 6 - 11

← 1 2 3 4 5 →