CircRRAS2 promotes myogenic differentiation of bovine MuSCs and is a novel regulatory molecule of muscle development

被引：0

作者：

An, Qiang ^{[1
]}

Zhang, Rui-Men ^{[1
]}

Wei, Yao ^{[1
]}

Zhang, Yong-Wang ^{[1
]}

Wang, Le-Yi ^{[1
]}

Ma, Shi-Nan ^{[2
]}

Zhang, Er-Kang ^{[1
]}

Zou, Chao-Xia ^{[1
]}

Yang, Su-Fang ^{[1
]}

Shi, De-Shun ^{[1
]}

Wei, Ying-Ming ^{[1
,3
]}

Deng, Yan-Fei ^{[1
,3
]}

机构：

[1] Guangxi Univ, Coll Anim Sci & Technol, Guangxi Key Lab Anim Breeding & Dis Control, Nanning, Peoples R China

[2] Hubei Univ Med, Tai He Hosp, Hubei Key Lab Embryon Stem Cell Res, Shiyan, Hubei, Peoples R China

[3] Guangxi Univ, Coll Anim Sci & Technol, Nanning, Guangxi, Peoples R China

来源：

ANIMAL BIOTECHNOLOGY | 2023年 / 34卷 / 09期

基金：

中国国家自然科学基金;

关键词：

CircRRAS2; muscle stem cells; myogenesis; differentiation; RNA-binding proteins;

D O I：

10.1080/10495398.2023.2196311

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The proliferation and myogenic differentiation of muscle stem cells (MuSCs) are important factors affecting muscle development and beef quality. There is increasing evidence that circRNAs can regulate myogenesis. We found a novel circRNA, named circRRAS2 that is significantly upregulated in the differentiation phase of bovine MuSCs. Here, we aimed to determine its roles in the proliferation and myogenic differentiation of these cells. The results showed that circRRAS2 was expressed in several bovine tissues. CircRRAS2 inhibited MuSCs proliferation and promoted myoblast differentiation. In addition, chromatin isolation by using RNA purification and mass spectrometry in differentiated muscle cells identified 52 RNA-binding proteins that could potentially bind to circRRAS2, in order to regulate their differentiation. The results suggest that circRRAS2 could be a specific regulator of myogenesis in bovine muscle.

引用

页码：4783 / 4792

页数：10

共 23 条

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[23] Tetrahydrogestrinone is an androgenic steroid that stimulates androgen receptor-mediated, myogenic differentiation in C3H10T1/2 multipotent mesenchymal cells and promotes muscle accretion in orchidectomized male rats
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