Selection and electrochemical-sensor application of an DNA-aptamer for methyl parathion detection

被引：22
|
作者：
Guo, Wenfei [1 ]
Hu, Changchun [1 ]
Li, Shuo [1 ]
Wei, Dan [1 ]
Zhou, Jie [1 ]
Liu, Xueying [1 ]
Chen, Hui [1 ]
Li, Song [1 ]
Deng, Yan [1 ]
机构：
[1] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Zhuzhou 412007, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2023年 / 1241卷
关键词：
Aptamer; Black phosphorus; SELEX; Electrochemical aptasensor; Methyl parathion; NANOCOMPOSITE; NANOPARTICLES; APTASENSOR;
D O I：
10.1016/j.aca.2023.340780
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An aptamer-based electrochemical sensor for methyl parathion (MP) detection is herein reported. The modified magnetic beads-systematic evolution of ligands by enrichment (MB-SELEX) was used to select the MP aptamer. After 14 rounds of selection, the aptamer (MPapta-6) with high affinity for MP was obtained, and its dissociation constant (Kd) was 39.66 +/- 14.73 mu M. Using the MPapta-6, the ultra-sensitive electrochemical sensor based on PLL-BP and AuNPs was constructed. The linear range of MP was 1-105 pM and detection limit (LOD) was as low as 0.49 pM. In addition, the application of the sensor in water samples was verified, and the recovery rate was 96.6%-103.5%. The results from this study showed that this strategy could be applied in practical detection.
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页数：7
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