Selection and electrochemical-sensor application of an DNA-aptamer for methyl parathion detection

被引：22

作者：

Guo, Wenfei ^{[1
]}

Hu, Changchun ^{[1
]}

Li, Shuo ^{[1
]}

Wei, Dan ^{[1
]}

Zhou, Jie ^{[1
]}

Liu, Xueying ^{[1
]}

Chen, Hui ^{[1
]}

Li, Song ^{[1
]}

Deng, Yan ^{[1
]}

机构：

[1] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Zhuzhou 412007, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2023年 / 1241卷

关键词：

Aptamer; Black phosphorus; SELEX; Electrochemical aptasensor; Methyl parathion; NANOCOMPOSITE; NANOPARTICLES; APTASENSOR;

D O I：

10.1016/j.aca.2023.340780

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An aptamer-based electrochemical sensor for methyl parathion (MP) detection is herein reported. The modified magnetic beads-systematic evolution of ligands by enrichment (MB-SELEX) was used to select the MP aptamer. After 14 rounds of selection, the aptamer (MPapta-6) with high affinity for MP was obtained, and its dissociation constant (Kd) was 39.66 +/- 14.73 mu M. Using the MPapta-6, the ultra-sensitive electrochemical sensor based on PLL-BP and AuNPs was constructed. The linear range of MP was 1-105 pM and detection limit (LOD) was as low as 0.49 pM. In addition, the application of the sensor in water samples was verified, and the recovery rate was 96.6%-103.5%. The results from this study showed that this strategy could be applied in practical detection.

引用

页数：7

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