Structure of the lipopolysaccharide O-antigen of endophytic Pseudomonas sp. strain L1

被引：0
|
作者：
Choma, Adam [1 ]
Kaczynski, Zbigniew [2 ]
Komaniecka, Iwona [1 ]
Swatek, Anita [1 ]
Kasperkiewicz, Katarzyna [3 ]
Pawlik, Malgorzata [3 ]
Piotrowska-Seget, Zofia [3 ]
机构：
[1] Marie Curie Sklodowska Univ, Inst Biol Sci, Dept Genet & Microbiol, Akad 19, PL-20033 Lublin, Poland
[2] Univ Gdansk, Fac Chem, Wita Stwosza 63, PL-80308 Gdansk, Poland
[3] Univ Silesia Katowice, Inst Biol Biotechnol & Environm Protect, Fac Nat Sci, Jagiellonska 28, PL-40032 Katowice, Poland
来源：
CARBOHYDRATE RESEARCH | 2023年 / 525卷
关键词：
Lipopolysaccharide; NMR; MALDI-TOF; O-specific polysaccharide; Endophytes; Pseudomonas sp; ENTEROBACTERIAL COMMON ANTIGEN; PETROLEUM-HYDROCARBONS; POLYSACCHARIDE; BACTERIA; MONOSACCHARIDES; CONFIGURATION; SPECTROSCOPY; XANTHOMONAS; DIVERSITY; LINKAGE;
D O I：
10.1016/j.carres.2023.108779
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Pseudomonas sp. Strain L1, the endophytic bacteria of Lolium perenne (ryegrass) plants growing in soil in an industrial area in the Silesia region (Zabrze, Southern Poland). The high-molecular-weight O-PS fraction liberated from Pseudomonas sp. L1 lipo-polysaccharide by mild acid hydrolysis was studied using chemical methods, MALDI-TOF mass spectrometry, and 1D and 2D NMR spectroscopy techniques. It was found that the O-specific polysaccharide was built of tetra-saccharide repeating units composed of D-FucpN, D-Fucp4N, and two D-QuipN residues. The following structure of the O-PS of Pseudomonas sp. Strain L1 was established:-> 4)-a-d-FucpN-(1 -> 3)-a-d-QuipN-(1 -> 3)-beta-d-QuipN-(1 -> 3)-a-d-Fucp4N-(1 ->
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页数：6
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