Green preparation of silver nanoparticles using leaf extract of Amoora rohituka for antioxidant, antibacterial and anticancer applications

Metal nanoparticles made from biological source are currently catching a lot of attention to the researcher because of their versatile application in different fields. Realizing this fact, the present study describe the utilization of Amoora rohituka leaves to prepare silver nanoparticles (AgNPs) and make their characterization along with evaluating bioactivities using different techniques. In UV-Vis spectrum, the highest absorption peak at 446 nm confirmed the formation of AgNPs whereas the FTIR spectrum exposed the participation of phytoconstituents with different functional group that play as reducing and capping agents in AgNPs synthesis. The face centered cubic structure with crystalline nature was found for AgNPs in XRD analysis and SEM captured the sphericalshaped images of nanoparticles with diameter ranging 5.63-23.6 nm. In antioxidant assay, the synthesized AgNPs exhibited prominent free radical scavenging capability for DPPH (IC50: 35.34 mu g/mL) and ABTS center dot + (IC50: 10.39 mu g/mL) radicals. The antibacterial efficiency of AgNPs can be ranked as Shigella boydii > Staphylococcus aureus > Escherichia coli. Moreover, the AgNPs displayed cytotoxicity against EAC cells in a dose-dependent pattern (IC50: 15.84 +/- 0.85 mu g/mL). The overall findings help to conclude that this green manufactured AgNPs might be a promising candidate for many biomedical applications.