In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma

被引：2
|
作者：
Zhang, Jie [1 ]
Lin, Xiao-Tong [1 ]
Fang, Lei [1 ]
Xie, Chuan-Ming [1 ]
机构：
[1] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Inst Hepatobiliary Surg,Key Lab Hepatobiliary & Pa, Chongqing 400038, Peoples R China
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 01期
基金：
中国国家自然科学基金;
关键词：
PROMOTION;
D O I：
10.1016/j.xpro.2023.102124
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
FBXO45, an E3 ubiquitin ligase highly expressed in liver tumors, is positively correlated with poor survival of hepatocellular carcinogenesis (HCC) patients, but whether FBXO45 drives HCC tumorigenesis remains largely unclear. Here, we describe a protocol that shortens the observation period for HCC tumorigenesis to assess the effects of FBXO45 in a DEN/CCl4-induced HCC mouse model. We describe steps for chemical induction of HCC in FBXO45-overexpressing mice, followed by tissue collection and pathology assessment via quantitative real-time PCR, histology, and immunohistochemistry.For complete details on the use and execution of this protocol, please refer to Lin et al. (2021).1
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页数：15
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