In vivo analysis of FBXO45-mediated fibrosis and liver tumorigenesis in a chemically induced mouse model of hepatocellular carcinoma

被引：2

作者：

Zhang, Jie ^{[1
]}

Lin, Xiao-Tong ^{[1
]}

Fang, Lei ^{[1
]}

Xie, Chuan-Ming ^{[1
]}

机构：

[1] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Inst Hepatobiliary Surg,Key Lab Hepatobiliary & Pa, Chongqing 400038, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

基金：

中国国家自然科学基金;

关键词：

PROMOTION;

D O I：

10.1016/j.xpro.2023.102124

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

FBXO45, an E3 ubiquitin ligase highly expressed in liver tumors, is positively correlated with poor survival of hepatocellular carcinogenesis (HCC) patients, but whether FBXO45 drives HCC tumorigenesis remains largely unclear. Here, we describe a protocol that shortens the observation period for HCC tumorigenesis to assess the effects of FBXO45 in a DEN/CCl4-induced HCC mouse model. We describe steps for chemical induction of HCC in FBXO45-overexpressing mice, followed by tissue collection and pathology assessment via quantitative real-time PCR, histology, and immunohistochemistry.For complete details on the use and execution of this protocol, please refer to Lin et al. (2021).1

引用

页数：15

共 39 条

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