Decorating phenylalanine side-chains with triple labeled 13C/19F/2H isotope patterns

被引：3

作者：

Toscano, Giorgia ^{[1
,3
]}

Holzinger, Julian ^{[2
]}

Nagl, Benjamin ^{[4
]}

Kontaxis, Georg ^{[5
]}

Kaehlig, Hanspeter ^{[4
]}

Konrat, Robert ^{[2
]}

Lichtenecker, Roman J. ^{[1
,6
]}

机构：

[1] Univ Vienna, Inst Organ Chem, Christian Doppler Lab High Content Struct Biol & B, Wahringer Str 38, A-1090 Vienna, Austria

[2] Univ Vienna, Dept Struct & Computat Biol, Christian Doppler Lab High Content Struct Biol & B, Max Perutz Labs, Campus Vienna Bioctr 5, A-1030 Vienna, Austria

[3] Univ Vienna, Vienna Doctoral Sch Chem DoSChem, Wahringer Str 42, A-1090 Vienna, Austria

[4] Univ Vienna, Inst Organ Chem, Wahringer Str 38, A-1090 Vienna, Austria

[5] Univ Vienna, Max Perutz Labs, Dept Struct & Computat Biol, Campus Vienna Bioctr 5, A-1030 Vienna, Austria

[6] MAG LAB, Karl Farkas Gasse 22, A-1030 Vienna, Austria

来源：

JOURNAL OF BIOMOLECULAR NMR | 2024年 / 78卷 / 03期

关键词：

Fluorine NMR; Fluorophenylalanine; Isotope labeling; F-TROSY; Protein overexpression; AMINO-ACIDS; F-19; NMR; RELAXATION; PROTEINS; TROSY;

D O I：

10.1007/s10858-024-00440-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present an economic and straightforward method to introduce C-13-F-19 spin systems into the deuterated aromatic side chains of phenylalanine as reporters for various protein NMR applications. The method is based on the synthesis of [4-C-13, 2,3,5,6-H-2(4)] 4-fluorophenylalanine from the commercially available isotope sources [2-C-13] acetone and deuterium oxide. This compound is readily metabolized by standard Escherichia coli overexpression in a glyphosate-containing minimal medium, which results in high incorporation rates in the corresponding target proteins.

引用

页码：139 / 147

页数：9

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