Rapid detection of varicella-zoster virus based on an immunochromatographic strip

被引：2

作者：

Wang, Aiping ^{[1
,2
]}

Niu, Yan ^{[2
]}

Zhao, Jianguo ^{[1
,3
]}

Liu, Hongliang ^{[1
,2
]}

Ding, Peiyang ^{[1
,2
]}

Chen, Yumei ^{[2
]}

Zhou, Jingming ^{[2
]}

Zhu, Xifang ^{[2
]}

Zhang, Ying ^{[2
]}

Liang, Chao ^{[2
]}

Zhang, Gaiping ^{[1
,2
,3
,4
]}

机构：

[1] Henan Longhu Modern Immun Lab, Zhengzhou, Henan, Peoples R China

[2] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Henan, Peoples R China

[3] Peking Univ, Coll Agr, Beijing, Peoples R China

[4] Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China

来源：

VIROLOGY | 2023年 / 586卷

关键词：

Varicella-zoster virus; Glycoprotein E; Immunochromatographic strip; Antigen detection; HERPES-SIMPLEX-VIRUS; PATHOGENESIS; INFECTIONS; VACCINE;

D O I：

10.1016/j.virol.2023.07.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Varicella-zoster virus (VZV) is a highly infectious DNA virus that can cause varicella (chickenpox) and herpes zoster (HZ). A simple, sensitive and specific detection method is desirable for the VZV infection. In this study, VZV gE protein, expressed in CHO cells, was used to immunize BALB/c mice for the generation of monoclonal antibodies (mAbs). For the first time, we developed a colloidal gold-based immunochromatographic strip for rapid detection of VZV using a pair of mAbs against gE protein. The limit of detection (LOD) of the strip was 30 ng mL-1 of purified VZV gE antigen, and it could specifically test VZV without cross-reactivity with Enterovirus 71 (EV-71), Herpes simplex virus 1 (HSV-1) and Herpes simplex virus 2 (HSV-2). The coincidence rate between the strip and commercial real-time PCR diagnostic kit was 100% using vesicle as the clinical sample. Our strip provided a technical support for rapid and specific detection of VZV.

引用

页码：35 / 42

页数：8

共 50 条

[21] Varicella-zoster virus and pregnancy
Charlier, Caroline
Le Mercier, Delphine
Salomon, Laurent J.
Ville, Yves
Kermorvant-Duchemin, Elsa
Frange, Pierre
Postaire, Martine
Lortholary, Olivier
Lecuit, Marc
Leruez-Ville, Marianne
PRESSE MEDICALE, 2014, 43 (06): : 665 - 675
[22] ANTIGENS OF VARICELLA-ZOSTER VIRUS
BRUNELL, PA
GRANAT, M
GERSHON, AA
JOURNAL OF IMMUNOLOGY, 1972, 108 (03): : 731 - &
[23] Varicella-Zoster Virus of the Eyelid
Watson, Alison H.
Homer, Natalie A.
Somogyi, Marie B.
JAMA OPHTHALMOLOGY, 2020, 138 (07) : 795 - 796
[24] INCUBATION OF VARICELLA-ZOSTER VIRUS
MYERS, MG
HIERHOLZER, WJ
AMERICAN JOURNAL OF DISEASES OF CHILDREN, 1984, 138 (12): : 1155 - 1156
[25] VARICELLA-ZOSTER VIRUS LATENCY
CROEN, KD
STRAUS, SE
ANNUAL REVIEW OF MICROBIOLOGY, 1991, 45 : 265 - 282
[26] ZOSTER AFTER EXPOSURE TO VARICELLA-ZOSTER VIRUS
MORENS, DM
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1985, 253 (12): : 1722 - 1723
[27] Varicella vaccine and infection with varicella-zoster virus
Vázquez, M
Shapiro, ED
NEW ENGLAND JOURNAL OF MEDICINE, 2005, 352 (05): : 439 - 440
[28] Varicella-zoster virus and virus DNA in the blood and oropharynx of people with latent or active varicella-zoster virus infections
Levin, Myron J.
JOURNAL OF CLINICAL VIROLOGY, 2014, 61 (04) : 487 - 495
[29] Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR
Weidmann, M
Meyer-König, U
Hufert, FT
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (04) : 1565 - 1568
[30] Varicella-Zoster Infection in Adult Males: Risk Factors for Varicella-Zoster Virus Pneumonia
Taha, Khaled Mohamed Hussain
El-Dein, Soheir Zein
Makboul, Gamal
KUWAIT MEDICAL JOURNAL, 2013, 45 (01): : 15 - 20

← 1 2 3 4 5 →