Particle finder: a simple particle detection tool for continuous-flow systems

被引：1
|
作者：
Fernandez-Mateo, Raul [1 ]
Calero, Victor [2 ]
Garcia-Sanchez, Pablo [3 ]
Ramos, Antonio [3 ]
Morgan, Hywel [1 ]
机构：
[1] Univ Southampton, Sch Elect & Comp Sci, Univ St, Southampton SO17 1BJ, England
[2] Int Iberian Nanotechnol Lab INL, Ave Mestre Jose Veiga S-N, P-4710 Braga, Portugal
[3] Univ Seville, Fac Fis, Dept Elect & Electromagnetismo, Avda Reina Mercedes S-N, Seville 41012, Spain
来源：
MICROFLUIDICS AND NANOFLUIDICS | 2023年 / 27卷 / 03期
关键词：
Continuous-flow microfluidics; Particle detection; GUI; Particle counting software; SEPARATION; CELLS;
D O I：
10.1007/s10404-023-02626-7
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
We describe a user-friendly, open source software for single-particle detection/counting in a continuous-flow. The tool automatically processes video images of particles, including pre-conditioning, followed by size-based discrimination for independent detection of fluorescent and non-fluorescent particles of different sizes. This is done by interactive tuning of a reduced set of parameters that can be checked with a robust, real-time quality control of the original video files. The software provides a concentration distribution of the particles in the transverse direction of the fluid flow. The software is a versatile tool for many microfluidic applications and does not require expertise in image analysis.
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页数：7
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